| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Armagan, Ilkay Bayram, Dilek Candan, Ibrahim Aydin Yigit, Ayse Celik, Erdal Armagan, Hamit Hakan Uguz, Abdülhadi Cihangir |
| Description |
Author Affiliation: Armagan I ( Department of Histology and Embryology, School of Medicine, Süleyman Demirel University, Isparta, Turkey. Electronic address: ilkayarmagan@sdu.edu.tr.); Bayram D ( Department of Histology and Embryology, School of Medicine, Süleyman Demirel University, Isparta, Turkey.); Candan IA ( Department of Histology and Embryology, School of Medicine, Süleyman Demirel University, Isparta, Turkey.); Yigit A ( Department of Medical Genetics, School of Medicine, Süleyman Demirel University, Isparta, Turkey.); Celik E ( Biochemistry Division, Isparta State Hospital, Isparta, Turkey.); Armagan HH ( Department of Emergency Medicine, School of Medicine, Süleyman Demirel University, Isparta, Turkey.); Uguz AC ( Department of Biophysics, School of Medicine, Süleyman Demirel University, Isparta, Turkey.) |
| Abstract |
The aim of the current study was to investigate the probable protective effects of Pentoxifylline (PTX) and Alpha Lipoic Acid (ALA), which display anti-oxidative efficacy against hepatotoxicity and nephrotoxicity, those being the major side effects of Methotrexate (MTX). Rats were divided into four groups: a control group; MTX (20mg/kg/day) group; MTX+PTX (20mg/kg/day+50mg/kg/day) group; and an MTX+ALA (20mg/kg/day+100mg/kg/day) group. At the end of the experiment, biochemical, histochemical and immunohistochemical analyses were performed on liver and kidney tissues of rats. We determined Glutathione Peroxidase (GSH-Px), Superoxide Dismutase (SOD), Catalase (CAT), Malondialdehyde (MDA), Nitric Oxide (NO) and Xanthine Oxidase (XO) levels in the liver and kidney. Moreover, Gamma Glutamyl Transferase (GGT), Direct Bilirubin (DBil), Blood Urea Nitrogen (BUN), and urea levels were measured in the serum. The histochemical evaluation revealed a significant decrease in MTX caused damage in the PTX- and ALA-treated groups (especially in ALA group). On the other hand, the immune staining of iNOS and TNF- were observed most densely in the MTX group, while the density decreased in the PTX- and ALA-administered groups. We determined increased GGT, BUN, urea and levels of CAT, MDA, NO, and XO values in both groups, while GSH-Px (an increase in liver tissue) and DBil levels were decreased in the group that received MTX. However, we determined decreased SOD levels in liver tissue. In the PTX and ALA groups, the levels of GGT, BUN and urea as well as the levels of CAT, MDA, NO and XO decreased (SOD increased in the liver tissue), and the levels of GSH-Px and DBil increased. In conclusion, it can be stated that, although ALA is more effective in preventing the toxic effects of MTX on the liver and kidney, PTX also has a preventive effect. As a result, we can readily suggest that ALA and PTX can have protective effects by decreasing MDA, NO, BUN and urea values as antioxidants against MTX-induced damage in liver and kidney of rats. |
| File Format |
HTM / HTML |
| ISSN |
13826689 |
| Issue Number |
3 |
| Volume Number |
39 |
| e-ISSN |
18727077 |
| Journal |
Environmental Toxicology and Pharmacology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-05-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Health Discipline Pharmacology Antioxidants Administration & Dosage Drug-induced Liver Injury Prevention & Control Kidney Diseases Methotrexate Adverse Effects Pentoxifylline Thioctic Acid Animals Pharmacology Biological Markers Blood Disease Models, Animal Gene Expression Regulation, Enzymologic Drug Effects Chemically Induced Male Rats Rats, Sprague-dawley Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
