| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Luan, Aina Tang, Futian Yang, Yuhong Lu, Meili Wang, Hongxin Zhang, Yingjie |
| Description |
Author Affiliation: Luan A ( Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China); Tang F ( Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.); Yang Y ( Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.); Lu M ( Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China.); Wang H ( Key Laboratory of Cardiovascular and Cerebrovascular Drug Research of Liaoning Province, Drug Research Institute, Liaoning Medical University, No. 40, Section 3, Songpo Road, Jinzhou 121001, PR China. Electronic address: hongxinwang@lnmu.edu.cn.); Zhang Y ( Internal Medicine-Cardiovascular Department, The First Affiliated Hospital of Liaoning Medical University, No. 2, Section 5, Renming Jie, Jinzhou 121001, PR China. Electronic address: zhangyingjiejinzhou@126.com.) |
| Abstract |
We previously reported that Astragalus polysaccharide (APS) extracted from Chinese medicine Astragalus membranaceus (Fisch.) Bge, attenuates hypertrophy of neonatal rat ventricular myocytes (NRVMs) induced by isoproterenol (Iso). The present study was designed to investigate the effects and the possible mechanism of APS on Iso-induced hypertrophy in rats and NRVMs with focus on tumor necrosis factor (TNF- )/peroxisome proliferator-activated receptor-γ coactivator 1 (PGC-1 ) signaling mediated energy biosynthesis. 36-Week old rats were randomly divided into 3 groups: (1) Control, rats received vehicle; (2) Iso, rats received isoproterenol injections; (3) Iso+APS, rats received isoproterenol injections and APS. NRVMs were divided into similar groups as rats. The results showed that combination of APS with Iso significantly attenuated the pathological changes, reduced the ratios of heart weight/body weight (HW/BW) and left ventricular weight/BW (LVW/BW), improved the cardiac hemodynamics, down-regulated mRNA and protein expression of atrial natriuretic peptide (ANP), increased the ratios of ATP/ADP and ATP/AMP, and decreased the content of free fatty acid (FFA) in heart tissue of rats compared with Iso alone. In addition, pretreatment with APS significantly decreased the surface area and protein content, down-regulated mRNA and protein expression of ANP, increased the ratios of ATP/ADP and ATP/AMP, and decreased the content of FFA in NRVMs compared with Iso alone. Furthermore, APS increased the protein expressions of ATP5D, the σ subunit of ATP synthase, PGC-1 and pyruvate dehydrogenase kinase 4 (PDK4) in tissue and NRVMs respectively and inhibited the production of TNF- in serum and culture medium compared with Iso alone. The results suggested that APS attenuates Iso-induced cardiac hypertrophy through regulating TNF- /PGC-1 signaling mediated energy biosynthesis. |
| File Format |
HTM / HTML |
| ISSN |
13826689 |
| Issue Number |
3 |
| Volume Number |
39 |
| e-ISSN |
18727077 |
| Journal |
Environmental Toxicology and Pharmacology |
| Language |
English |
| Publisher |
Elsevier |
| Publisher Date |
2015-05-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Environmental Health Discipline Pharmacology Astragalus Membranaceus Chemistry Cardiomegaly Prevention & Control Energy Metabolism Drug Effects Isoproterenol Adverse Effects Polysaccharides Administration & Dosage Signal Transduction Animals Animals, Newborn Chemically Induced Cells, Cultured Drugs, Chinese Herbal Pharmacology Gene Expression Regulation Injections Male Rats Transcription Factors Genetics Metabolism Tumor Necrosis Factor-alpha Journal Article Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Health, Toxicology and Mutagenesis Medicine Toxicology Pharmacology |
