NDLI: Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons

Content Provider	PubMed Central
Author	Liu, Shan Wang, Xing Yun, Li Lei, Xu Yu, Xiaoliang Lin, Ge Jun, Li Zhu, Yongjin Sudan, He
Copyright Year	2014
Abstract	Tumor necrosis factor-α (TNF-α) is a critical proinflammatory cytokine regulating neuroinflammation. Elevated levels of TNF-α have been associated with various neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease. However, the signaling events that lead to TNF-α-initiated neurotoxicity are still unclear. Here, we report that RIP3-mediated necroptosis, a form of regulated necrosis, is activated in the mouse hippocampus after intracerebroventricular injection of TNF-α. RIP3 deficiency attenuates TNF-α-initiated loss of hippocampal neurons. Furthermore, we characterized the molecular mechanism of TNF-α-induced neurotoxicity in HT-22 hippocampal neuronal cells. HT-22 cells are sensitive to TNF-α only upon caspase blockage and subsequently undergo necrosis. The cell death is suppressed by knockdown of CYLD or RIP1 or RIP3 or MLKL, suggesting that this necrosis is necroptosis and mediated by CYLD-RIP1-RIP3-MLKL signaling pathway. TNF-α-induced necroptosis of HT-22 cells is largely independent of both ROS accumulation and calcium influx although these events have been shown to be critical for necroptosis in certain cell lines. Taken together, these data not only provide the first in vivo evidence for a role of RIP3 in TNF-α-induced toxicity of hippocampal neurons, but also demonstrate that TNF-α promotes CYLD-RIP1-RIP3-MLKL-mediated necroptosis of hippocampal neurons largely bypassing ROS accumulation and calcium influx.
Related Links	http://dx.doi.org/10.1155/2014/290182
Starting Page	290182
File Format	PDF
ISSN	23146141
e-ISSN	23146141
Journal	BioMed Research International
Volume Number	2014
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2014-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Structure-function analysis of an evolutionary conserved protein, DAP3, which mediates TNF-alpha- and Fas-induced cell death.

Hippocampal neurons wait their turn

Rhythmic coordination of hippocampal neurons during associative memory processing

Early presynaptic changes during plasticity in cultured hippocampal neurons

Gene-gun Transfection of Hippocampal Neurons

Calcium Phosphate Transfection of Primary Hippocampal Neurons

VTA neurons coordinate with the hippocampal reactivation of spatial experience

Nuclear export of phosphorylated C/EBPβ mediates the inhibition of albumin expression by TNF-α

Hippocampal neurons recycle BDNF for activity-dependent secretion and LTP maintenance

Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons

Similar Documents

Structure-function analysis of an evolutionary conserved protein, DAP3, which mediates TNF-alpha- and Fas-induced cell death.

Hippocampal neurons wait their turn

Rhythmic coordination of hippocampal neurons during associative memory processing

Early presynaptic changes during plasticity in cultured hippocampal neurons

Gene-gun Transfection of Hippocampal Neurons

Calcium Phosphate Transfection of Primary Hippocampal Neurons

VTA neurons coordinate with the hippocampal reactivation of spatial experience

Nuclear export of phosphorylated C/EBPβ mediates the inhibition of albumin expression by TNF-α

Hippocampal neurons recycle BDNF for activity-dependent secretion and LTP maintenance

Necroptosis Mediates TNF-Induced Toxicity of Hippocampal Neurons