NDLI: Effects of Growth Phase and Temperature on σ B Activity within a Listeria monocytogenes Population: Evidence for RsbV-Independent Activation of σ B at Refrigeration Temperatures

Content Provider	PubMed Central
Author	Marta, Utratna Cosgrave, Eoin Baustian, Claas Ceredig, Rhodri H. O'byrne, Conor P.
Copyright Year	2014
Abstract	The alternative sigma factor σ B of Listeria monocytogenes is responsible for regulating the transcription of many of the genes necessary for adaptation to both food-related stresses and to conditions found within the gastrointestinal tract of the host. The present study sought to investigate the influence of growth phase and temperature on the activation of σ B within populations of L. monocytogenes EGD-e wild-type, ΔsigB, and ΔrsbV throughout growth at both 4°C and 37°C, using a reporter fusion that couples expression of EGFP to the strongly σ B-dependent promoter of lmo2230. A similar σ B activation pattern within the population was observed in wt-egfp at both temperatures, with the highest induction of σ B occurring in the early exponential phase of growth when the fluorescent population rapidly increased, eventually reaching the maximum in early stationary phase. Interestingly, induction of σ B activity was heterogeneous, with only a proportion of the cells in the wt-egfp population being fluorescent above the background autofluorescence level. Moreover, significant RsbV-independent activation of σ B was observed during growth at 4°C. This result suggests that an alternative route to σ B activation exists in the absence of RsbV, a finding that is not explained by the current model for σ B regulation.
Related Links	http://dx.doi.org/10.1155/2014/641647
Starting Page	641647
File Format	PDF
ISSN	23146133
e-ISSN	23146141
Journal	BioMed Research International
Volume Number	2014
Language	English
Publisher	Hindawi Publishing Corporation
Publisher Date	2014-01-01
Access Restriction	Open
Rights Holder	Hindawi Publishing Corporation
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Immunology and Microbiology Medicine Biochemistry, Genetics and Molecular Biology

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