NDLI: Development of Schwann cell-seeded conduit using chitosan-based biopolymers for nerve repair

Content Provider	IEEE Xplore Digital Library
Author	Khataokar, A. Skop, N. Haesun Kim Pfister, B. Cho, C.H.
Copyright Year	2010
Description	Author affiliation: Department of Biomedical Engineering, New Jersey Institute of Technology, Newark, NJ 07102 (Khataokar, A.; Skop, N.; Pfister, B.; Cho, C.H.) \|\| Department of Biology, Rutgers University, Newark, NJ 07102 (Haesun Kim)
Abstract	The purpose of this study is to develop biodegradable nerve guidance conduits seeded with Schwann cells (SC) using chitosan-based biopolymers for improving nerve regeneration. Chitosan, prepared from alkaline hydrolysis of chitin, is a natural polysaccharide biopolymer, having biocompatibility and biodegradability. In this study, chitosan and chitosan-gelatin membranes were prepared, characterized, and evaluated. The SC isolated from rat sciatic nerve was seeded on chitosan and chitosan-gelatin membranes to investigate their interactions. To enhance the SC attachment and growth on the membranes, we incorporated the poly-L-lysine (PLL) into chitosan and chitosan-gelatin network at various ratios of 1:800, 1:200, 1:50 (chitosan or chitosan-gelatin: PLL). We have also investigated the effect of extracellular matrix (ECM) molecules on the proliferation of SC. We observed that SC proliferation on PLL-coated surface was significantly higher than that of ECM coated and uncoated surfaces. With the increase of PLL ratio into chitosan or chitosan-gelatin network, the attachment and growth of SCs were significantly enhanced. This study indicates that the incorporation of PLL into chitosan and chitosan-gelatin membranes significantly improves the bioactivity of the materials. The Schwann cell-seeded films can be used to construct a three-dimensional nerve conduit for enhancing nerve regeneration.
Starting Page	1
Ending Page	2
File Size	474289
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458176
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Phase locked loops Biomembranes Electrochemical machining Surface morphology Cells (biology) Regeneration engineering Biomedical engineering Biodegradable materials Extracellular Biological materials
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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