NDLI: Three-dimensional liver cell cultures enhance urea synthesis and drug metabolism

Content Provider	IEEE Xplore Digital Library
Author	Roy, D. Wei Sun
Copyright Year	2010
Description	Author affiliation: Drexel University, 3141 Chestnut Street, Philadelphia, PA 19104 (Roy, D.; Wei Sun)
Abstract	Tissue engineering within three-dimensional scaffolds represents a developing field with the potential to create in vitro livers. Human liver cells, HepG2, respond to cues in their immediate microenvironment by modulating cell functions including cell proliferation, urea synthesis and drug metabolism. The importance of three-dimensional environments draws from the fact that it has been extensively used for guided tissue regeneration. HepG2 cells grown in traditional two-dimensional supports were compared to three-dimensional cultures in polystyrene scaffolds. The 5-day experimental study examined the effects of the different environments on HepG2 cell urea synthesis using a quantitative colorimetric assay and drug metabolism-specific function using the cytochrome P450 monooxygenase substrate, 7-ethoxy-4-trifluoromethyl-coumarin. The function of liver cells grown on three-dimensional scaffolds was enhanced compared to the activity of cells grown on two-dimensional supports. The results suggest that HepG2 liver cell function in three-dimensional environments more closely mimic physiological responses than existing two-dimensional culture systems.
Starting Page	1
Ending Page	2
File Size	134588
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458258
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Liver Drugs Biochemistry Cells (biology) In vitro Biological materials Biological tissues Humans Tissue engineering Electrochemical machining
Content Type	Text
Resource Type	Article

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