NDLI: A biphasic electrical stimulation enhanced cellular activity on the cell-based olfactory biosensor

Content Provider	IEEE Xplore Digital Library
Author	Jeong, S. H. Lee, S. H. Park, T. H. Kim, S. J.
Copyright Year	2010
Description	Author affiliation: Interdisciplinary Program in Brain Science, Seoul National University, Seoul, Korea (151-742) (Kim, S. J.) \|\| Corporate R&D Institute, Samsung Electro-Mechanics, Suwon, Korea (443-743) (Jeong, S. H.) \|\| School of Chemical and Biological Engineering, Seoul National University, Seoul, Korea (151-742) (Lee, S. H.; Park, T. H.)
Abstract	Recently, we have researched that the binding of olfactory receptors with odorant molecules initiates olfactory signal transduction, which leads to a cationic influx on Microelectode arrays. Human embryonic kidney-293 cells were recombinantly engineered to co-express olfactory receptors I7 and gustatory cyclic nucleotide gated channels, which increased the cationic influx. The odorant-induced change in the membrane potential was measured in the extracellular region using microelectrode arrays. In the present study, a biphasic electrical current stimulation was applied to the cells in order to increase the intrinsic cellular activity. Upon odorants recognition, the electrical cellular activity was enhanced following each electrical stimulation pulse. Compared with odorant-only stimulation, the electrical stimulation induced to an increase in the amplitude and frequency of the electrical signal, which was produced in response to the odorants. These results demonstrate that electrical stimulation can enhance cellular activity and increase the response signal for detecting ligand binding. This paradigm of electrical stimulation can be used as a novel method in the field of versatile cell-based biosensors for detection of specific odorants.
Starting Page	1
Ending Page	2
File Size	1076041
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458285
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Olfactory Electrical stimulation Biosensors Electric potential Biomembranes Humans Extracellular Olfactory receptor Cell-based olfactory biosensor Cellular activity Microelectrode arrays
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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