NDLI: Effect of wear-debris particles on RAW 264.7 cells

Content Provider	IEEE Xplore Digital Library
Author	Musib, M.K. Saha, S.
Copyright Year	2010
Description	Author affiliation: Department of Orthopedic Surgery and Rehabilitation Medicine, SUNY Downstate Medical Center, Brooklyn, New York 11203 (Musib, M.K.; Saha, S.)
Abstract	Wear-debris particles from ultrahigh molecular weight polyethylene (UHMWPE) and metals has been implicated in periprosthetic osteolysis and cellular response to them depend on their size and dose. Response to larger particles has been previously studied, but there are no specific studies examining biological response to simultaneous administration of both UHMWPE and Ti particles less than 0.2μm in size. Our lab has developed a standardized and reproducible technique to isolate, characterize and fractionate both particles into 3 distinct size ranges [1.0–10.0μm (micron), 0.2–1.0μm (submicron) and 0.01–0.2μm (nano)] and furthermore minimize clumping, which facilitated disaggregation and isolation of individual particles. RAW 264.7 cells were treated for 24h, 48h and 72h with various doses and sizes of both particulates simultaneously. Cell membrane damage was measured by quantifying lactate dehydrogenase (LDH) release. The cells elicited a dose and size dependent response to the nano-particulates at the highest dose. At 72h there was a significant increase in LDH released by cells treated with the nanoparticles as compared to untreated controls as well as to larger particles. To mimic in vivo conditions, further studies are being conducted and will involve administration of dual wear particulates that will help understand cellular response to wear particulates.
Starting Page	1
Ending Page	2
File Size	254536
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458278
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Fractionation Cells (biology) Biomembranes Analysis of variance Polyethylene Nanobioscience Nanoparticles Suspensions Filters Orthopedic surgery
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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