NDLI: Effect of hydroxyapatite particles on stem cell response in nanofiber scaffolds

Content Provider	IEEE Xplore Digital Library
Author	Kwei, S.P. Moffat, K.L. Doty, S. Lu, H.H.
Copyright Year	2010
Description	Author affiliation: Department of Biomedical Engineering, Columbia University (Kwei, S.P.; Moffat, K.L.; Lu, H.H.) \|\| Orthopaedic Surgery, Hospital for Special Surgery, NY, NY (Doty, S.)
Abstract	Rotator cuff tears are among the most common shoulder injuries that require surgery. High failure rates of biological graft-based repairs underscore the need for functional alternatives. Specifically, functional grafts must incorporate the gradient of mineralization from tendon to bone in order to be biomimetic. In this study, effects of varying concentrations of mineral content in aligned nanofiber scaffolds on human mesenchymal stem cells (hMSC) are evaluated. It is hypothesized that mineral content will regulate cell response, matrix deposition, and integrin gene expression. hMSC were seeded on aligned nanofiber scaffolds of polylactide-co-glycolide with 0%, 10%, and 15% hydroxyapatite content, and were maintained in chondrogenic medium. Cell proliferation (n=5), collagen deposition (n=5), and gene expression (n=5) for Collagen X, Sox9, osteopontin, osteonectin, and osteocalcin were determined over 42 days. Cell number was found to differ between the non-mineralized and mineralized groups. Significant increase in collagen deposition over time was observed in mineralized scaffold groups, and the 15% group showing significantly higher deposition than the 0% group by day 42. Lower expressions of chondrocyte hyperotrophy marker Collagen X and chondrogenic marker Sox9 and maintained high expressions of osteogenic markers osteopontin, osteonectin, and osteocalcin suggest an osteogenic lineage for the stem cells. In conclusion, addition of HA particles influenced hMSC proliferation, matrix deposition, and may induce an osteogenic differentiation response from the stem cells.
Starting Page	1
Ending Page	2
File Size	322947
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458123
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Stem cells Nanobioscience Mineralization Minerals Gene expression Injuries Surgery Tendons Bones Biomimetics
Content Type	Text
Resource Type	Article

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