NDLI: Patterning micro-stiffness in cell-adhesive substrate using microfluidics-based lithography

Content Provider	IEEE Xplore Digital Library
Author	Yuk Kee Cheung Azeloglu, E.U. Shiovitz, D. Costa, K.D. Seliktar, D. Sia, S.K.
Copyright Year	2010
Description	Author affiliation: Biomedical Engineering Department, Technion-Israel Institute of Technology, Technion City, Haifa 32000, Israel (Seliktar, D.) \|\| Department of Biomedical Engineering, Columbia University, 351 Engineering Terrace, 1210 Amsterdam Avenue, New York, NY 10027, USA (Yuk Kee Cheung; Azeloglu, E.U.; Shiovitz, D.; Costa, K.D.; Sia, S.K.)
Abstract	In this study, we demonstrate the formation of 3D cell-adhesive hydrogels exhibiting well-defined spatial variation in stiffness using our previously developed microfluidics-based lithography technique [12]. PEG monoacrylate-linked bovine fibrinogen (PEG-fibrinogen) is photopolymerized into specific, user-defined shapes inside a microchannel, and successive cycles of fabrication result in a heterogeneous structure with controlled regional variations in stiffness. Atomic force microscope (AFM) indentations were used to directly confirm the micro-stiffness distribution, and morphological and migratory behavior of cells in microenvironments of controlled variations in stiffness was characterized. Our approach allows control of microscale variations of stiffness in cell-adhesive substrates with high precision and flexibility and offers the opportunity to examine differential cell-ECM interactions relevant to a multitude of fundamental cellular processes [14].
Starting Page	1
Ending Page	2
File Size	543617
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458111
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Lithography Atomic force microscopy Biomedical engineering Microfluidics Shape control In vitro Image analysis Analysis of variance Area measurement Image segmentation
Content Type	Text
Resource Type	Article

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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