NDLI: Encapsulation within nanofibers confers stability to the protective antigen protein

Content Provider	IEEE Xplore Digital Library
Author	Knockenhauer, K.E. Sawicka, K.M. Simon, S.R.
Copyright Year	2010
Description	Author affiliation: Stony Brook University Basic Science Tower 9-146 Stony Brook, NY 11794-8691 (Knockenhauer, K.E.; Sawicka, K.M.; Simon, S.R.)
Abstract	The current vaccination paradigm for the prevention of anthrax is insufficient to deal with a potential, widespread epidemic. To solve this issue, we propose a self-administrable vaccine patch capable of delivering the antigen of interest into the skin. This patch is comprised of solid-state nanofibers containing encapsulated protective antigen (PA), a binding protein secreted by Bacillus anthracis. Polyvinylpyrrolidone (PVP) nanofibers, produced by the electrospinning technique, are utilized as our transdermal delivery vector because the high surface area to volume ratio that they afford maximizes contact with the skin; in turn increasing local concentration gradients of reversibly packaged PA when the hygroscopic PVP is solubilized via transepidermal water loss. Previous studies have confirmed the retention of PA immunoreactivity and functionality after the voltage-intensive electrospinning process. The study described here aimed to compare the retention of PA functionality within the nanofibers to PA in solution over a several month period; since it is theorized that encapsulation within nanofibers may confer protein stability. The functionality of encapsulated PA was retained whereas PA in solution was inactive after a 32 week incubation at 4°C; suggesting that encapsulation within nanofibers confers stability to the PA protein.
Starting Page	1
Ending Page	2
File Size	169466
Page Count	2
File Format	PDF
ISBN	9781424468799
e-ISBN	9781424469246
DOI	10.1109/NEBC.2010.5458205
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2010-03-26
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Encapsulation Stability Protection Proteins Skin Nanobioscience Needles Vaccines Immune system Voltage
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
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9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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