NDLI: Cytokine secretion by CD4+ T cells at the immunological synapse requires Cdc42-dependent local actin remodeling but not microtubule organizing center polarity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Dogniaux, Stéphanie Guégan, Sarah Miro, Francesc Bohineust, Armelle Chemin, Karine Hivroz, Claire Tourret, Marie
Description	Country affiliation: France Author Affiliation: Chemin K ( Institut Curie, Centre de Recherche, Paris F-75248, France.)
Abstract	Cytokine secretion by T lymphocytes plays a central role in mounting adaptive immune responses. However, little is known about how newly synthesized cytokines, once produced, are routed within T cells and about the mechanisms involved in regulating their secretions. In this study, we investigated the role of cytoskeleton remodeling at the immunological synapse (IS) in cytokine secretion. We show that a key regulator of cytoskeleton remodeling, the Rho GTPase Cdc42, controls IFN-Î³ secretion by primary human CD4+ T lymphocytes. Surprisingly, microtubule organizing center polarity at the IS, which does not depend on Cdc42, is not required for cytokine secretion by T lymphocytes, whereas microtubule polymerization is required. In contrast, actin remodeling at the IS, which depends on Cdc42, controls the formation of the polymerized actin ring at the IS, the dynamic concentration of IFN-Î³-containing vesicles inside this ring, and the secretion of these vesicles. These results reveal a previously unidentified role of Cdc42-dependent actin remodeling in cytokine exocytosis at the IS.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	5
Volume Number	189
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2012-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Actins Metabolism Cd4-positive T-lymphocytes Immunology Secretion Cell Polarity Cytokines Immunological Synapses Microtubule-organizing Center Cdc42 Gtp-binding Protein Physiology Antagonists & Inhibitors Deficiency Cytology Cell Line, Transformed Coculture Techniques Exocytosis Hek293 Cells Jurkat Cells Polymerization Primary Cell Culture Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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