NDLI: Acquisition of MHC:peptide complexes by dendritic cells contributes to the generation of antiviral CD8+ T cell immunity in vivo.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lombardi, Giovanna Klavinskis, Linda S. Hervouet, Catherine Lechler, Robert I. Ellis, Richard Smyth, Lesley A. Becker, Pablo D. Hayday, Thomas
Description	Country affiliation: United kingdom Author Affiliation: Smyth LA ( Medical Research Council Centre for Transplantation, King's College London, London SE1 9RT, United Kingdom. lesley.smyth@kcl.ac.uk)
Abstract	There is an increasing body of evidence suggesting that the transfer of preformed MHC class I:peptide complexes between a virus-infected cell and an uninfected APC, termed cross-dressing, represents an important mechanism of Ag presentation to CD8+ T cells in host defense. However, although it has been shown that memory CD8+ T cells can be activated by uninfected dendritic cells (DCs) cross-dressed by Ag from virus-infected parenchymal cells, it is unknown whether conditions exist during virus infection in which naive CD8+ T cells are primed and differentiate to cytolytic effectors through cross-dressing, and indeed which DC subset would be responsible. In this study, we determine whether the transfer of MHC class I:peptide complexes between infected and uninfected murine DC plays a role in CD8+ T cell priming to viral Ags in vivo. We show that MHC class I:peptide complexes from peptide-pulsed or virus-infected DCs are indeed acquired by splenic CD8 â » DCs in vivo. Furthermore, the acquired MHC class I:peptide complexes are functional in that they induced Ag-specific CD8+ T cell effectors with cytolytic function. As CD8 â » DCs are poor cross-presenters, this may represent the main mechanism by which CD8 â » DCs present exogenously encountered Ag to CD8+ T cells. The sharing of Ag as preformed MHC class I:peptide complexes between infected and uninfected DCs without the restraints of Ag processing may have evolved to accurately amplify the response and also engage multiple DC subsets critical in the generation of strong antiviral immunity.
ISSN	00221767
e-ISSN	15506606
Journal	The Journal of Immunology
Issue Number	5
Volume Number	189
Language	English
Publisher	The American Association of Immunologists
Publisher Date	2012-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Antiviral Agents Metabolism Cd8-positive T-lymphocytes Virology Dendritic Cells Immunity, Cellular Major Histocompatibility Complex Immunology Peptide Fragments Adenoviridae Adoptive Transfer Animals Cells, Cultured Cross-priming Mice Mice, Inbred Balb C Mice, Inbred C57bl Mice, Knockout Mice, Transgenic Ovalbumin Administration & Dosage Biosynthesis Research Support, Non-u.s. Gov't Discipline Immunology
Content Type	Text
Resource Type	Article
Subject	Immunology and Allergy Immunology

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