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Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis.

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Effect of ATRA on the expression of HOXA5 gene in K562 cells and its relationship with cell cycle and apoptosis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liu, Chun-Yan Liu, Wen-Jun Bai, Yong-Qi Zhang, Teng Guo, Qu-Lian
Description	Author Affiliation: Liu WJ ( Department of Pediatrics, Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 64600, P.R. China.); Zhang T ( Department of Pediatrics, Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 64600, P.R. China.); Guo QL ( Department of Pediatrics, Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 64600, P.R. China.); Liu CY ( Department of Pediatrics, Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 64600, P.R. China.); Bai YQ ( Department of Pediatrics, Affiliated Hospital of Sichuan Medical University, Luzhou, Sichuan 64600, P.R. China.)
Abstract	Leukemia is the most common malignant disease in children with high incidence and mortality rates, and a poor treatment effect. The aim of the present study was to examine the changes in the expression of homeobox (Hox) A5 gene and its relationship with cell cycle and apoptosis through the intervention of human K562 myeloid leukemia cell line by all-trans retinoic acid (ATRA), to analyze the role of HOXA5 in the pathogenesis and development process of myeloid leukemia. The optimal concentration of ATRA to be used with K562 cells was determined using a cell counting kit8 (CCK8). After 24, 72 and 48 h following treatment of K562 cells with 10 µmol/l ATRA, cell cycle events and apoptosis were measured using flow cytometry. HOXA5 mRNA and protein expression in K562 cells was assessed by RTPCR and western blot analysis, and the relationship between HOXA5 expression and cell cycle and apoptosis was analyzed. The HOXA5 mRNA and protein expression levels were increased following treatment with ATRA in K562 cells. Apoptosis was increased significantly. The cell cycle was inhibited in G0/G1 phase. Cell proliferation was also inhibited. HOXA5 mRNA and protein expression rates positively correlated with cell apoptosis and the increased percentage and cell cycle of the G0/G1 phase. However, HOXA5 negatively correlated with the reduced percentage of S stage. In conclusion, the expression of HOXA5 in cells was increased following treatment with ATRA in K562 cells, in a time-dependent manner. Additionally, ATRA may inhibit the proliferation of K562 cells and promote apoptosis by upregulating the HOXA5 mRNA and protein expression.
ISSN	17912997
e-ISSN	17913004
DOI	10.3892/mmr.2016.5086
Journal	Molecular Medicine Reports
Issue Number	5
Volume Number	13
Language	English
Publisher	Spandidos Publications
Publisher Date	2016-05-01
Publisher Place	Greece
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology
Content Type	Text
Resource Type	Article
Subject	Genetics Biochemistry Molecular Biology Cancer Research Molecular Medicine Oncology

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