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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Su, Y. Q. Niu, S. F. Qiu, F. Liu, M. Chen, X. Wang, J. Zhong, S. P. |
| Description | Country affiliation: China Author Affiliation: Chen X ( Department of Marine technology, College of Ocean, Nantong University, Nantong, China.) |
| Abstract | In order to investigate the immune role of ribosomal protein L10 (RPL10/QM-like gene) in marine fish, we challenged the large yellow croaker Pseudosciaena (= Larimichthys) crocea, the most important marine fish culture species in China, by injection with a mixture of the bacteria Vibrio harveyi and V. parahaemolyticus (3:1 in volume). Microarray analysis and real-time PCR were performed 24 and 48 h post-challenge to isolate and identify the QM-like gene from the gill P. crocea (designated PcQM). The expression level of the PcQM gene did not changed significantly at 24 h post-challenge, but was significantly downregulated at 48 h post-challenge, suggesting that the gene had an immune-modulatory effect in P. crocea. Full-length PcQM cDNA and genomic sequences were obtained by rapid amplification of cDNA ends (RACE)-PCR. The sequence of the PcQM gene clustered together with those of other QM-like genes from other aquatic organisms, indicating that the QM-like gene is highly conserved in teleosts. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2012-10-15 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Immunity Genetics Perciformes Ribosomal Proteins Isolation & Purification Amino Acid Sequence Animals Dna, Complementary Gene Expression Regulation Genome Gills Metabolism Microbiology Molecular Sequence Data Oligonucleotide Array Sequence Analysis Phylogeny Chemistry Sequence Alignment Vibrio Physiology Vibrio Infections Immunology Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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