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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Zhang, Xiao Wei, Wei Wu, Haizhen Xu, Hongyan Chang, Kangmei Zhang, Yuanxing |
| Description | Author Affiliation: Zhang X ( State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, PR China.) |
| Abstract | Ferritin, an iron-binding protein, plays a role in host immune response to invading stimulus. Two ferritin subunit cDNAs, lycfh and lycfm, were identified from large yellow croaker, Pseudosciaena crocea. Analysis of the lycfh and lycfm 5'-UTR showed that both had a putative iron responsive element (IRE). Amino acid sequence alignment and phylogenetic analysis indicated that LycfH and LycfM had high identity to H and M type subunit of vertebrate ferritins respectively, while both of them showed the low identity to L type subunit. In all eight tissues examined, lycfh and lycfm expressions were detectable on transcriptional level. Real-time PCR assay indicated that the transcriptional levels of lycfh and lycfm were significantly increased in liver, spleen and kidney of the fish stimulated by attenuated live Vibrio anguillarum strain. Furthermore, ferritin subunit H and M were probably two candidates of immune molecules involved in acute phase response in large yellow croaker because their transcriptional levels were quickly increased and reached the peak levels at 12 h in liver after challenged with bacteria. |
| File Format | HTM / HTML |
| ISSN | 10504648 |
| Issue Number | 5-6 |
| Volume Number | 28 |
| e-ISSN | 10959947 |
| Journal | Fish & Shellfish Immunology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2010-05-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Marine Biology Discipline Immunology Apoferritins Genetics Immunology Ferritins Gene Expression Regulation Perciformes Amino Acid Sequence Animals Chemistry Base Sequence Cloning, Molecular Fish Diseases Gene Expression Profiling Kidney Liver Molecular Sequence Data Classification Phylogeny Sequence Alignment Spleen Vibrio Vibrio Infections Veterinary Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Environmental Chemistry Medicine Aquatic Science Immunology |
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