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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Semir, J. Oliveira, V. M. Mansanares, M. E. Forni-Martins, E. R. |
| Description | Country affiliation: Brazil Author Affiliation: Oliveira VM ( Laboratório de Biossistemática e Evolução, Departamento de Biologia Vegetal, Instituto de Biologia, Universidade Estadual de Campinas, Campinas, SP, Brasil.) |
| Abstract | In order to extend our knowledge concerning karyotypes of the genus Vernonia, we applied various techniques of chromosome banding, including AgNOR and triple staining with the fluorochromes CMA/DA/DAPI (CDD), and of fluorescent in situ hybridization (FISH) for the 45S rDNA probe to specimens of two populations of Vernonia geminata collected from an open-pasture area, in southern Brazil. B chromosomes were observed in one of the populations. Both populations of V. geminata presented a pair of CMA(3)(+) terminal bands and one pair of chromosomes with terminal AgNOR banding. The FISH evidenced, in one population, two pairs of small sites of 45S rDNA; these being two small terminal sites and two centromeric sites. In the other population, there was only one pair of small terminal sites and two sites in two B chromosomes, one in each chromosome. There was coincidence of localization between CMA(+) and NOR bands with one of the small terminal sites of 45S rDNA of one chromosome of the normal complement, but not in B chromosomes. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2012-12-06 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Karyotype Vernonia Genetics Chromosome Banding Chromosomes, Plant Dna, Ribosomal Fluorescent Dyes Chemistry Genes, Plant In Situ Hybridization, Fluorescence Indoles Silver Staining Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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