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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Tang, S. Rehana, B. Bao, E. D. Lv, Y. J. Yue, Z. H. Islam, A. Liu, Z. J. Zhang, M. Hartung, J. |
| Description | Country affiliation: China Author Affiliation: Liu ZJ ( Animal Pathology Laboratory, Department of Basic Veterinary, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, China.) |
| Abstract | We investigated and described the kinetics of heat shock protein (Hsp) 110 expression and distribution in rat primary myocardial cells exposed to heat stress in vitro. After incubation at 37°C for 72 h, myocardial cells were heat stressed at 42°C for 0, 10, 20, 40, 60, 120, 240, 360, and 480 min. Significant increases in aspartate transaminase, lactate dehydrogenase, and creatine kinase enzymatic activities in the myocardial cell culture media were observed during heat stress, suggesting that the integrity of the myocardial cells was altered. Immunocytochemical analysis revealed that the expressed Hsp110 was constitutively localized in the cytoplasm and in the nuclei in small amounts characterized by a granular pattern. Nuclear Hsp110 levels increased significantly after 240 min of heat stress compared with levels in the control. The overall levels of Hsp110 expression increased significantly after 20 min. After 240 min, Hsp110 levels were approximately 1.2-fold higher than those in the control. Increasing levels of hsp110 messenger RNA detected using real-time quantitative polymerase chain reaction were observed after 20 min of heat stress, and the levels peaked with a 10-fold increase after 240 min of heat stress. These results indicate that the expression of Hsp110 in primary myocardial cells in vitro is sensitive to hyperthermic stress and that Hsp110 is involved in the potential acquisition of thermotolerance after heat stress. Therefore, Hsp110 might play a fundamental role in opposing and alleviating heat-induced damage caused by hyperthermic stress in primary myocardial cells. |
| e-ISSN | 16765680 |
| Journal | Genetics and Molecular Research |
| Issue Number | 4 |
| Volume Number | 11 |
| Language | English |
| Publisher | Fundação de Pesquisas Científicas de Ribeirão Preto |
| Publisher Date | 2012-11-29 |
| Publisher Place | Brazil |
| Access Restriction | Open |
| Subject Keyword | Gene Expression Regulation Hsp110 Heat-shock Proteins Metabolism Myocytes, Cardiac Animals Aspartate Aminotransferases Cells, Cultured Creatine Kinase Culture Media, Conditioned Gene Expression Genetics Heat-shock Response Kinetics L-lactate Dehydrogenase Secretion Primary Cell Culture Rna, Messenger Research Support, Non-u.s. Gov't Discipline Genetics Discipline Molecular Biology Discipline Bioinformatics |
| Content Type | Text |
| Resource Type | Article |
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