NDLI: Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

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Year: 2013 Issue: 82

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Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

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Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Chung, Wen Yuan Eltweri, Amar M. Isherwood, John Haqq, Jonathan Ong, Seok Ling Gravante, Gianpiero Lloyd, David M. Metcalfe, Matthew S. Dennison, Ashley R.
Description	Author Affiliation: Chung WY ( Department of Hepatobiliary and Pancreatic Surgery, University Hospitals of Leicester.)
Abstract	The use of ex vivo perfused models can mimic the physiological conditions of the liver for short periods, but to maintain normal homeostasis for an extended perfusion period is challenging. We have added the kidney to our previous ex vivo perfused liver experiment model to reproduce a more accurate physiological state for prolonged experiments without using live animals. Five intact livers and kidneys were retrieved post-mortem from sacrificed pigs on different days and perfused for a minimum of 6 hr. Hourly arterial blood gases were obtained to analyze pH, lactate, glucose and renal parameters. The primary endpoint was to investigate the effect of adding one kidney to the model on the acid base balance, glucose, and electrolyte levels. The result of this liver-kidney experiment was compared to the results of five previous liver only perfusion models. In summary, with the addition of one kidney to the ex vivo liver circuit, hyperglycemia and metabolic acidosis were improved. In addition this model reproduces the physiological and metabolic responses of the liver sufficiently accurately to obviate the need for the use of live animals. The ex vivo liver-kidney perfusion model can be used as an alternative method in organ specific studies. It provides a disconnection from numerous systemic influences and allows specific and accurate adjustments of arterial and venous pressures and flow.
File Format	HTM / HTML
e-ISSN	1940087X
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-18
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Kidney Physiology Liver Perfusion Animals Extracorporeal Membrane Oxygenation In Vitro Techniques Blood Supply Metabolism Organ Preservation Swine Video-audio Media
Content Type	Text
Resource Type	Article

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