NDLI: Implanting glass spinal cord windows in adult mice with experimental autoimmune encephalomyelitis.

Please wait, while we are loading the content...

Year: 2013 Issue: 82

An orthotopic bladder cancer model for gene delivery studies.

High-resolution, high-speed, three-dimensional video imaging with digital fringe projection techniques.

The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation

Monolayer contact doping of silicon surfaces and nanowires using organophosphorus compounds.

Dual-phase cone-beam computed tomography to see, reach, and treat hepatocellular carcinoma during drug-eluting beads transarterial chemo-embolization.

Production and purification of non replicative canine adenovirus type 2 derived vectors.

Measurement of coherence decay in GaMnAs using femtosecond four-wave mixing.

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Enrichment and purging of human embryonic stem cells by detection of cell surface antigens using the monoclonal antibodies TG30 and GCTM-2.

Rapid generation of amyloid from native proteins in vitro.

Measuring sensitivity to viewpoint change with and without stereoscopic cues.

Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response.

Quantitative analysis and characterization of atherosclerotic lesions in the murine aortic sinus.

Use of an eight-arm radial water maze to assess working and reference memory following neonatal brain injury.

Identifying protein-protein interaction in Drosophila adult heads by Tandem Affinity Purification (TAP).

Microfluidic picoliter bioreactor for microbial single-cell analysis: fabrication, system setup, and operation.

Using an automated 3D-tracking system to record individual and shoals of adult zebrafish.

MRI and PET in mouse models of myocardial infarction.

Evaluating plasmonic transport in current-carrying silver nanowires.

Analysis of apoptosis in zebrafish embryos by whole-mount immunofluorescence to detect activated Caspase 3.

Collecting saliva and measuring salivary cortisol and alpha-amylase in frail community residing older adults via family caregivers.

Computerized dynamic posturography for postural control assessment in patients with intermittent claudication.

Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor.

Cell-based flow cytometry assay to measure cytotoxic activity.

Implanting glass spinal cord windows in adult mice with experimental autoimmune encephalomyelitis.

Measuring changes in tactile sensitivity in the hind paw of mice using an electronic von Frey apparatus.

In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors.

Localization and relative quantification of carbon nanotubes in cells with multispectral imaging flow cytometry.

Monitoring the assembly of a secreted bacterial virulence factor using site-specific crosslinking.

Using Caenorhabditis elegans as a model system to study protein homeostasis in a multicellular organism.

Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

Methods for facilitating microbial growth on pulp mill waste streams and characterization of the biodegradation potential of cultured microbes.

Establishment of an in vitro system to study intracellular behavior of Candida glabrata in human THP-1 macrophages.

Visualizing cytoplasmic flow during single-cell wound healing in Stentor coeruleus.

Concurrent quantification of cellular and extracellular components of biofilms.

The cell-based L-glutathione protection assays to study endocytosis and recycling of plasma membrane proteins.

Surface renewal: an advanced micrometeorological method for measuring and processing field-scale energy flux density data.

Methods to identify the NMR resonances of the ¹³C-dimethyl N-terminal amine on reductively methylated proteins.

Simultaneous multicolor imaging of biological structures with fluorescence photoactivation localization microscopy.

Analysis of skeletal muscle defects in larval zebrafish by birefringence and touch-evoke escape response assays.

A fluorescence-based exonuclease assay to characterize DmWRNexo, orthologue of human progeroid WRN exonuclease, and its application to other nucleases.

Investigating receptor-ligand systems of the cellulosome with AFM-based single-molecule force spectroscopy.

Seeded synthesis of CdSe/CdS rod and tetrapod nanocrystals.

Generation of lymph node-fat pad chimeras for the study of lymph node stromal cell origin.

High throughput microfluidic rapid and low cost prototyping packaging methods.

Isolation of viable multicellular glands from tissue of the carnivorous plant, Nepenthes.

Structure and coordination determination of peptide-metal complexes using 1D and 2D ¹H NMR.

Helminth collection and identification from wildlife.

Sex stratified neuronal cultures to study ischemic cell death pathways.

Characteristics of precipitation-formed polyethylene glycol microgels are controlled by molecular weight of reactants.

An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.

Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Preparation of silica nanoparticles through microwave-assisted acid-catalysis.

In situ TEM of biological assemblies in liquid.

V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting.

Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice.

Corticospinal excitability modulation during action observation.

Nest building as an indicator of health and welfare in laboratory mice.

PLGA nanoparticles formed by single- or double-emulsion with vitamin E-TPGS.

Intravital video microscopy measurements of retinal blood flow in mice.

P50 sensory gating in infants.

A dual tracer PET-MRI protocol for the quantitative measure of regional brain energy substrates uptake in the rat.

Identification of specific sensory neuron populations for study of expressed ion channels.

Waste water derived electroactive microbial biofilms: growth, maintenance, and basic characterization.

A high-throughput-compatible FRET-based platform for identification and characterization of botulinum neurotoxin light chain modulators.

Laser-scanning photostimulation of optogenetically targeted forebrain circuits.

Whole-cell MALDI-TOF mass spectrometry is an accurate and rapid method to analyze different modes of macrophage activation.

Year: 2013 Issue: 81

Year: 2013 Issue: 80

Year: 2013 Issue: 79

Year: 2013 Issue: 78

Year: 2013 Issue: 77

Year: 2013 Issue: 76

Year: 2013 Issue: 75

Year: 2013 Issue: 74

Year: 2013 Issue: 73

Year: 2013 Issue: 72

Year: 2013 Issue: 71

Implanting glass spinal cord windows in adult mice with experimental autoimmune encephalomyelitis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fenrich, Keith K. Weber, Pascal Rougon, Genevieve Debarbieux, Franck
Description	Author Affiliation: Fenrich KK ( Developmental Biology Institute of Marseille-Luminy (IBDML), Aix Marseille University.)
Abstract	Experimental autoimmune encephalomyelitis (EAE) in adult rodents is the standard experimental model for studying autonomic demyelinating diseases such as multiple sclerosis. Here we present a low-cost and reproducible glass window implantation protocol that is suitable for intravital microscopy and studying the dynamics of spinal cord cytoarchitecture with subcellular resolution in live adult mice with EAE. Briefly, we surgically expose the vertebrae T12-L2 and construct a chamber around the exposed vertebrae using a combination of cyanoacrylate and dental cement. A laminectomy is performed from T13 to L1, and a thin layer of transparent silicone elastomer is applied to the dorsal surface of the exposed spinal cord. A modified glass cover slip is implanted over the exposed spinal cord taking care that the glass does not directly contact the spinal cord. To reduce the infiltration of inflammatory cells between the window and spinal cord, anti-inflammatory treatment is administered every 2 days (as recommended by ethics committee) for the first 10 days after implantation. EAE is induced only 2-3 weeks after the cessation of anti-inflammatory treatment. Using this approach we successfully induced EAE in 87% of animals with implanted windows and, using Thy1-CFP-23 mice (blue axons in dorsal spinal cord), quantified axonal loss throughout EAE progression. Taken together, this protocol may be useful for studying the recruitment of various cell populations as well as their interaction dynamics, with subcellular resolution and for extended periods of time. This intravital imaging modality represents a valuable tool for developing therapeutic strategies to treat autoimmune demyelinating diseases such as EAE.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50826
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-21
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Encephalomyelitis, Autoimmune, Experimental Pathology Microscopy, Fluorescence, Multiphoton Prostheses And Implants Spinal Cord Animals Surgery Mice Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in