NDLI: Quantitative analysis and characterization of atherosclerotic lesions in the murine aortic sinus.

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Year: 2013 Issue: 82

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Quantitative analysis and characterization of atherosclerotic lesions in the murine aortic sinus.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Venegas-Pino, Daniel E. Banko, Nicole Khan, Mohammed I. Shi, Yuanyuan Werstuck, Geoff H.
Description	Author Affiliation: Venegas-Pino DE ( Department of Biochemistry and Biomedical Sciences, McMaster University.)
Abstract	Atherosclerosis is a disease of the large arteries and a major underlying cause of myocardial infarction and stroke. Several different mouse models have been developed to facilitate the study of the molecular and cellular pathophysiology of this disease. In this manuscript we describe specific techniques for the quantification and characterization of atherosclerotic lesions in the murine aortic sinus and ascending aorta. The advantage of this procedure is that it provides an accurate measurement of the cross-sectional area and total volume of the lesion, which can be used to compare atherosclerotic progression across different treatment groups. This is possible through the use of the valve leaflets as an anatomical landmark, together with careful adjustment of the sectioning angle. We also describe basic staining methods that can be used to begin to characterize atherosclerotic progression. These can be further modified to investigate antigens of specific interest to the researcher. The described techniques are generally applicable to a wide variety of existing and newly created dietary and genetically-induced models of atherogenesis.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50933
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-07
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Atherosclerosis Pathology Sinus Of Valsalva Animals Aorta Metabolism Disease Models, Animal Immunohistochemistry Mice Staining And Labeling Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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