NDLI: Technique of subnormothermic ex vivo liver perfusion for the storage, assessment, and repair of marginal liver grafts.

Please wait, while we are loading the content...

Real-time impedance-based cell analyzer as a tool to delineate molecular pathways involved in neurotoxicity and neuroprotection in a neuronal cell line.

Network analysis of the default mode network using functional connectivity MRI in Temporal Lobe Epilepsy.

Micro-masonry for 3D additive micromanufacturing.

Stab wound injury of the zebrafish adult telencephalon: a method to investigate vertebrate brain neurogenesis and regeneration.

Sonication-facilitated immunofluorescence staining of late-stage embryonic and larval Drosophila tissues in situ.

Single plane illumination module and micro-capillary approach for a wide-field microscope.

Measuring respiratory function in mice using unrestrained whole-body plethysmography.

Design and Construction of an Urban Runoff Research Facility

Measurement of greenhouse gas flux from agricultural soils using static chambers.

High-throughput assay to phenotype Salmonella enterica Typhimurium association, invasion, and replication in macrophages.

Real-time imaging of single engineered RNA transcripts in living cells using ratiometric bimolecular beacons.

Modeling astrocytoma pathogenesis in vitro and in vivo using cortical astrocytes or neural stem cells from conditional, genetically engineered mice.

A mouse model for pathogen-induced chronic inflammation at local and systemic sites.

A video protocol of retroviral infection in primary intestinal organoid culture.

Ultrasound-guided transthoracic intramyocardial injection in mice.

Real-time imaging of endothelial cell-cell junctions during neutrophil transmigration under physiological flow.

Isolation of blood-vessel-derived multipotent precursors from human skeletal muscle.

Zinc-finger nuclease enhanced gene targeting in human embryonic stem cells.

Organotypic slice cultures to study oligodendrocyte dynamics and myelination.

A lateralized odor learning model in neonatal rats for dissecting neural circuitry underpinning memory formation.

Using fluorescent proteins to monitor glycosome dynamics in the African trypanosome.

Subtype-selective electroporation of cortical interneurons.

Olfactory assays for mouse models of neurodegenerative disease.

Isolation of murine lymph node stromal cells.

Bladder smooth muscle strip contractility as a method to evaluate lower urinary tract pharmacology.

Technique of subnormothermic ex vivo liver perfusion for the storage, assessment, and repair of marginal liver grafts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Knaak, Jan M. Spetzler, Vinzent N. Goldaracena, Nicolas Louis, Kristine S. Selzner, Nazia Selzner, Markus
Description	Author Affiliation: Knaak JM ( Multi Organ Transplant Program, Toronto General Hospital); Spetzler VN ( Multi Organ Transplant Program, Toronto General Hospital); Goldaracena N ( Multi Organ Transplant Program, Toronto General Hospital); Louis KS ( Multi Organ Transplant Program, Toronto General Hospital); Selzner N ( Multi Organ Transplant Program, Toronto General Hospital); Selzner M ( Multi Organ Transplant Program, Toronto General Hospital)
Abstract	The success of liver transplantation has resulted in a dramatic organ shortage. In most transplant regions 20-30% of patients on the waiting list for liver transplantation die without receiving an organ transplant or are delisted for disease progression. One strategy to increase the donor pool is the utilization of marginal grafts, such as fatty livers, grafts from older donors, or donation after cardiac death (DCD). The current preservation technique of cold static storage is only poorly tolerated by marginal livers resulting in significant organ damage. In addition, cold static organ storage does not allow graft assessment or repair prior to transplantation. These shortcomings of cold static preservation have triggered an interest in warm perfused organ preservation to reduce cold ischemic injury, assess liver grafts during preservation, and explore the opportunity to repair marginal livers prior to transplantation. The optimal pressure and flow conditions, perfusion temperature, composition of the perfusion solution and the need for an oxygen carrier has been controversial in the past. In spite of promising results in several animal studies, the complexity and the costs have prevented a broader clinical application so far. Recently, with enhanced technology and a better understanding of liver physiology during ex vivo perfusion the outcome of warm liver perfusion has improved and consistently good results can be achieved. This paper will provide information about liver retrieval, storage techniques, and isolated liver perfusion in pigs. We will illustrate a) the requirements to ensure sufficient oxygen supply to the organ, b) technical considerations about the perfusion machine and the perfusion solution, and c) biochemical aspects of isolated organs.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/51419
Journal	Journal of Visualized Experiments
Issue Number	90
Language	English
Publisher	MyJove Corp.
Publisher Date	2014-08-13
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Liver Transplantation Liver Organ Preservation Perfusion Animals Cold Temperature Models, Animal Swine Tissue Donors Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation.

Subnormothermic machine perfusion for non-heart-beating donor liver grafts preservation in a Swine model: a new strategy to increase the donor pool?

Subnormothermic Ex Vivo Lung Perfusion Temperature Improves Graft Preservation in Lung Transplantation

Ex Situ Normothermic Machine Perfusion of Donor Livers.

Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Editorial: Ex vivo Liver Machine Perfusion

Eight-Hour Continuous Normothermic Ex Vivo Kidney Perfusion Is a Safe Preservation Technique for Kidney Transplantation: A New Opportunity for the Storage, Assessment, and Repair of Kidney Grafts.

Attempt to rescue discarded human liver grafts by end ischemic hypothermic oxygenated machine perfusion.

Technique of subnormothermic ex vivo liver perfusion for the storage, assessment, and repair of marginal liver grafts.

Similar Documents

Normothermic Ex Vivo Kidney Perfusion for the Preservation of Kidney Grafts prior to Transplantation.

Subnormothermic machine perfusion for non-heart-beating donor liver grafts preservation in a Swine model: a new strategy to increase the donor pool?

Subnormothermic Ex Vivo Lung Perfusion Temperature Improves Graft Preservation in Lung Transplantation

Ex Situ Normothermic Machine Perfusion of Donor Livers.

Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts

Editorial: Ex vivo Liver Machine Perfusion

Eight-Hour Continuous Normothermic Ex Vivo Kidney Perfusion Is a Safe Preservation Technique for Kidney Transplantation: A New Opportunity for the Storage, Assessment, and Repair of Kidney Grafts.

Attempt to rescue discarded human liver grafts by end ischemic hypothermic oxygenated machine perfusion.

Technique of subnormothermic ex vivo liver perfusion for the storage, assessment, and repair of marginal liver grafts.