NDLI: High throughput microfluidic rapid and low cost prototyping packaging methods.

Please wait, while we are loading the content...

Year: 2013 Issue: 82

An orthotopic bladder cancer model for gene delivery studies.

High-resolution, high-speed, three-dimensional video imaging with digital fringe projection techniques.

The Utility of Stage-specific Mid-to-late Drosophila Follicle Isolation

Monolayer contact doping of silicon surfaces and nanowires using organophosphorus compounds.

Dual-phase cone-beam computed tomography to see, reach, and treat hepatocellular carcinoma during drug-eluting beads transarterial chemo-embolization.

Production and purification of non replicative canine adenovirus type 2 derived vectors.

Measurement of coherence decay in GaMnAs using femtosecond four-wave mixing.

Simultaneous Electrophysiological Recording and Calcium Imaging of Suprachiasmatic Nucleus Neurons

Enrichment and purging of human embryonic stem cells by detection of cell surface antigens using the monoclonal antibodies TG30 and GCTM-2.

Rapid generation of amyloid from native proteins in vitro.

Measuring sensitivity to viewpoint change with and without stereoscopic cues.

Novel whole-tissue quantitative assay of nitric oxide levels in Drosophila neuroinflammatory response.

Quantitative analysis and characterization of atherosclerotic lesions in the murine aortic sinus.

Use of an eight-arm radial water maze to assess working and reference memory following neonatal brain injury.

Identifying protein-protein interaction in Drosophila adult heads by Tandem Affinity Purification (TAP).

Microfluidic picoliter bioreactor for microbial single-cell analysis: fabrication, system setup, and operation.

Using an automated 3D-tracking system to record individual and shoals of adult zebrafish.

MRI and PET in mouse models of myocardial infarction.

Evaluating plasmonic transport in current-carrying silver nanowires.

Analysis of apoptosis in zebrafish embryos by whole-mount immunofluorescence to detect activated Caspase 3.

Collecting saliva and measuring salivary cortisol and alpha-amylase in frail community residing older adults via family caregivers.

Computerized dynamic posturography for postural control assessment in patients with intermittent claudication.

Nonhuman primate lung decellularization and recellularization using a specialized large-organ bioreactor.

Cell-based flow cytometry assay to measure cytotoxic activity.

Implanting glass spinal cord windows in adult mice with experimental autoimmune encephalomyelitis.

Measuring changes in tactile sensitivity in the hind paw of mice using an electronic von Frey apparatus.

In vivo reprogramming of adult somatic cells to pluripotency by overexpression of Yamanaka factors.

Localization and relative quantification of carbon nanotubes in cells with multispectral imaging flow cytometry.

Monitoring the assembly of a secreted bacterial virulence factor using site-specific crosslinking.

Using Caenorhabditis elegans as a model system to study protein homeostasis in a multicellular organism.

Steps for the autologous ex vivo perfused porcine liver-kidney experiment.

Methods for facilitating microbial growth on pulp mill waste streams and characterization of the biodegradation potential of cultured microbes.

Establishment of an in vitro system to study intracellular behavior of Candida glabrata in human THP-1 macrophages.

Visualizing cytoplasmic flow during single-cell wound healing in Stentor coeruleus.

Concurrent quantification of cellular and extracellular components of biofilms.

The cell-based L-glutathione protection assays to study endocytosis and recycling of plasma membrane proteins.

Surface renewal: an advanced micrometeorological method for measuring and processing field-scale energy flux density data.

Methods to identify the NMR resonances of the ¹³C-dimethyl N-terminal amine on reductively methylated proteins.

Simultaneous multicolor imaging of biological structures with fluorescence photoactivation localization microscopy.

Analysis of skeletal muscle defects in larval zebrafish by birefringence and touch-evoke escape response assays.

A fluorescence-based exonuclease assay to characterize DmWRNexo, orthologue of human progeroid WRN exonuclease, and its application to other nucleases.

Investigating receptor-ligand systems of the cellulosome with AFM-based single-molecule force spectroscopy.

Seeded synthesis of CdSe/CdS rod and tetrapod nanocrystals.

Generation of lymph node-fat pad chimeras for the study of lymph node stromal cell origin.

High throughput microfluidic rapid and low cost prototyping packaging methods.

Isolation of viable multicellular glands from tissue of the carnivorous plant, Nepenthes.

Structure and coordination determination of peptide-metal complexes using 1D and 2D ¹H NMR.

Helminth collection and identification from wildlife.

Sex stratified neuronal cultures to study ischemic cell death pathways.

Characteristics of precipitation-formed polyethylene glycol microgels are controlled by molecular weight of reactants.

An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.

Optimized protocol for retinal wholemount preparation for imaging and immunohistochemistry.

A Rapid Protocol for Integrating Extrachromosomal Arrays With High Transmission Rate into the C. elegans Genome

Preparation of silica nanoparticles through microwave-assisted acid-catalysis.

In situ TEM of biological assemblies in liquid.

V3 stain-free workflow for a practical, convenient, and reliable total protein loading control in western blotting.

Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice.

Corticospinal excitability modulation during action observation.

Nest building as an indicator of health and welfare in laboratory mice.

PLGA nanoparticles formed by single- or double-emulsion with vitamin E-TPGS.

Intravital video microscopy measurements of retinal blood flow in mice.

P50 sensory gating in infants.

A dual tracer PET-MRI protocol for the quantitative measure of regional brain energy substrates uptake in the rat.

Identification of specific sensory neuron populations for study of expressed ion channels.

Waste water derived electroactive microbial biofilms: growth, maintenance, and basic characterization.

A high-throughput-compatible FRET-based platform for identification and characterization of botulinum neurotoxin light chain modulators.

Laser-scanning photostimulation of optogenetically targeted forebrain circuits.

Whole-cell MALDI-TOF mass spectrometry is an accurate and rapid method to analyze different modes of macrophage activation.

Year: 2013 Issue: 81

Year: 2013 Issue: 80

Year: 2013 Issue: 79

Year: 2013 Issue: 78

Year: 2013 Issue: 77

Year: 2013 Issue: 76

Year: 2013 Issue: 75

Year: 2013 Issue: 74

Year: 2013 Issue: 73

Year: 2013 Issue: 72

Year: 2013 Issue: 71

High throughput microfluidic rapid and low cost prototyping packaging methods.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Miled, Amine Sawan, Mohamad
Description	Author Affiliation: Miled A ( Electrical Engineering Department, Polytechnique Montreal.)
Abstract	In this work, 3 different packaging and assembly techniques are presented. They can be classified into two categories: one-time use and reusable packaging techniques. The one-time use packaging technique employs UV-based and temperature curing epoxies to connect microtubes to access holes, wire-bonding for integrated circuit connections, and silver epoxy for electrical connections. This method is based on a robust assembly technique that can support relatively high pressure close to 1 psi and does not need any support to strengthen the microfluidic architecture. Reusable packaging techniques consist of PDMS-based microtube interconnectors and anisotropic adhesive films for electrical connections. These devices are more sensitive and fragile. Consequently, Plexiglas support is added to the microfluidic structure to improve the electrical contact when anisotropic adhesive films are used, and also to strengthen the microfluidic architecture. In addition, a micromanipulator is needed to maintain tubes while using a thin PDMS layer to connect them to the access holes. Different PDMS layer thicknesses, ranging from 0.45-3 mm, are tested to compare the best adherence versus injection rates. Applied injection rates are varied from 50-300 µl/hr for 0.45-3 mm PDMS layers, respectively. These techniques are mainly applicable for low-pressure applications. However, they can be extended for high-pressure ones through plasma-oxygen process to permanently seal the PDMS to glass substrates. The main advantage of this technique, besides the fact that it is reusable, consists of keeping the device observable when the microchannel length is very short (in the range of 3 mm or lower).
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50735
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-23
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine High-throughput Screening Assays Instrumentation Microfluidic Analytical Techniques Dimethylpolysiloxanes Chemistry Epoxy Compounds Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in