NDLI: An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.

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Year: 2013 Issue: 82

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An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.

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An in vitro model to study immune responses of human peripheral blood mononuclear cells to human respiratory syncytial virus infection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Vissers, Marloes Habets, Marrit N. Ahout, Inge M. L. Jans, Jop de Jonge, Marien I. Diavatopoulos, Dimitri A. Ferwerda, Gerben
Description	Author Affiliation: Vissers M ( Laboratory of Pediatric Infectious Diseases, Department of Pediatrics, Radboud university medical center.)
Abstract	Human respiratory syncytial virus (HRSV) infections present a broad spectrum of disease severity, ranging from mild infections to life-threatening bronchiolitis. An important part of the pathogenesis of severe disease is an enhanced immune response leading to immunopathology. Here, we describe a protocol used to investigate the immune response of human immune cells to an HRSV infection. First, we describe methods used for culturing, purification and quantification of HRSV. Subsequently, we describe a human in vitro model in which peripheral blood mononuclear cells (PBMCs) are stimulated with live HRSV. This model system can be used to study multiple parameters that may contribute to disease severity, including the innate and adaptive immune response. These responses can be measured at the transcriptional and translational level. Moreover, viral infection of cells can easily be measured using flow cytometry. Taken together, stimulation of PBMC with live HRSV provides a fast and reproducible model system to examine mechanisms involved in HRSV-induced disease.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50766
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-10
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Leukocytes, Mononuclear Immunology Virology Respiratory Syncytial Virus Infections Respiratory Syncytial Viruses Hela Cells Blood Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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