NDLI: Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice.

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Year: 2013 Issue: 82

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Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice.

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Marble burying and nestlet shredding as tests of repetitive, compulsive-like behaviors in mice.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Angoa-Pérez, Mariana Kane, Michael J. Briggs, Denise I. Francescutti, Dina M. Kuhn, Donald M.
Description	Author Affiliation: Angoa-Pérez M ( Research and Development Service, John D. Dingell VA Medical Center and Department of Psychiatry & Behavioral Neurosciences, Wayne State University School of Medicine.)
Abstract	Obsessive-compulsive disorder (OCD) and autism spectrum disorders (ASD) are serious and debilitating psychiatric conditions and each constitutes a significant public health concern, particularly in children. Both of these conditions are highlighted by the repeated expression of meaningless behaviors. Individuals with OCD often show checking, frequent hand washing, and counting. Children with ASDs also engage in repetitive tapping, arm or hand flapping, and rocking. These behaviors can vary widely in intensity and frequency of expression. More intense forms of repetitive behaviors can even result in injury (e.g. excessive grooming, hand washing, and self-stimulation). These behaviors are therefore very disruptive and make normal social discourse difficult. Treatment options for repetitive behaviors in OCD and ASDs are somewhat limited and there is great interest in developing more effective therapies for each condition. Numerous animal models for evaluating compulsive-like behaviors have been developed over the past three decades. Perhaps the animal models with the greatest validity and ease of use are the marble burying test and the nestlet shredding test. Both tests take advantage of the fact that the target behaviors occur spontaneously in mice. In the marble burying test, 20 marbles are arrayed on the surface of clean bedding. The number of marbles buried in a 30 min session is scored by investigators blind to the treatment or status of the subjects. In the nestlet shredding test, a nestlet comprised of pulped cotton fiber is preweighed and placed on top of cage bedding and the amount of the nestlet remaining intact after a 30 min test session is determined. Presently, we describe protocols for and show movie documentation of marble burying and nestlet shredding. Both tests are easily and accurately scored and each is sensitive to small changes in the expression of compulsive-like behaviors that result from genetic manipulations, disease, or head injury.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50978
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-24
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Behavior, Animal Compulsive Behavior Disease Models, Animal Nesting Behavior Obsessive-compulsive Disorder Animals Mice Research Support, N.i.h., Extramural Research Support, U.s. Gov't, Non-p.h.s. Video-audio Media
Content Type	Text
Resource Type	Article

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