NDLI: Using an automated 3D-tracking system to record individual and shoals of adult zebrafish.

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Year: 2013 Issue: 82

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Using an automated 3D-tracking system to record individual and shoals of adult zebrafish.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Maaswinkel, Hans Zhu, Liqun Weng, Wei
Description	Author Affiliation: Maaswinkel H ( R&D, xyZfish.)
Abstract	Like many aquatic animals, zebrafish (Danio rerio) moves in a 3D space. It is thus preferable to use a 3D recording system to study its behavior. The presented automatic video tracking system accomplishes this by using a mirror system and a calibration procedure that corrects for the considerable error introduced by the transition of light from water to air. With this system it is possible to record both single and groups of adult zebrafish. Before use, the system has to be calibrated. The system consists of three modules: Recording, Path Reconstruction, and Data Processing. The step-by-step protocols for calibration and using the three modules are presented. Depending on the experimental setup, the system can be used for testing neophobia, white aversion, social cohesion, motor impairments, novel object exploration etc. It is especially promising as a first-step tool to study the effects of drugs or mutations on basic behavioral patterns. The system provides information about vertical and horizontal distribution of the zebrafish, about the xyz-components of kinematic parameters (such as locomotion, velocity, acceleration, and turning angle) and it provides the data necessary to calculate parameters for social cohesions when testing shoals.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50681
Journal	Journal of Visualized Experiments
Issue Number	82
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-12-05
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Imaging, Three-dimensional Instrumentation Video Recording Zebrafish Physiology Animals Behavior, Animal Drug Effects Dizocilpine Maleate Pharmacology Video-audio Media
Content Type	Text
Resource Type	Article

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