NDLI: Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.

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Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.

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Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Qi, Guanxiao Radnikow, Gabriele Feldmeyer, Dirk
Description	Author Affiliation: Qi G ( Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich.); Radnikow G ( Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich.); Feldmeyer D ( Institute of Neuroscience and Medicine (INM-2), Research Centre Jülich)
Abstract	The combination of patch clamp recordings from two (or more) synaptically coupled neurons (paired recordings) in acute brain slice preparations with simultaneous intracellular biocytin filling allows a correlated analysis of their structural and functional properties. With this method it is possible to identify and characterize both pre- and postsynaptic neurons by their morphology and electrophysiological response pattern. Paired recordings allow studying the connectivity patterns between these neurons as well as the properties of both chemical and electrical synaptic transmission. Here, we give a step-by-step description of the procedures required to obtain reliable paired recordings together with an optimal recovery of the neuron morphology. We will describe how pairs of neurons connected via chemical synapses or gap junctions are identified in brain slice preparations. We will outline how neurons are reconstructed to obtain their 3D morphology of the dendritic and axonal domain and how synaptic contacts are identified and localized. We will also discuss the caveats and limitations of the paired recording technique, in particular those associated with dendritic and axonal truncations during the preparation of brain slices because these strongly affect connectivity estimates. However, because of the versatility of the paired recording approach it will remain a valuable tool in characterizing different aspects of synaptic transmission at identified neuronal microcircuits in the brain.
File Format	HTM / HTML
e-ISSN	1940087X
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-10
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Brain Physiology Nerve Net Neurons Patch-clamp Techniques Animals Axons Dendrites Gap Junctions Mice Synapses Synaptic Transmission Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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