NDLI: Measurement of the pressure-volume curve in mouse lungs.

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Measurement of the pressure-volume curve in mouse lungs.

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Measurement of the pressure-volume curve in mouse lungs.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Limjunyawong, Nathachit Fallica, Jonathan Horton, Maureen R. Mitzner, Wayne
Description	Author Affiliation: Limjunyawong N ( Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University.); Fallica J ( Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University.); Horton MR ( Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University.); Mitzner W ( Environmental Health Sciences, Bloomberg School of Public Health, Johns Hopkins University)
Abstract	In recent decades the mouse has become the primary animal model of a variety of lung diseases. In models of emphysema or fibrosis, the essential phenotypic changes are best assessed by measurement of the changes in lung elasticity. To best understand specific mechanisms underlying such pathologies in mice, it is essential to make functional measurements that can reflect the developing pathology. Although there are many ways to measure elasticity, the classical method is that of the total lung pressure-volume (PV) curve done over the whole range of lung volumes. This measurement has been made on adult lungs from nearly all mammalian species dating back almost 100 years, and such PV curves also played a major role in the discovery and understanding of the function of pulmonary surfactant in fetal lung development. Unfortunately, such total PV curves have not been widely reported in the mouse, despite the fact that they can provide useful information on the macroscopic effects of structural changes in the lung. Although partial PV curves measuring just the changes in lung volume are sometimes reported, without a measure of absolute volume, the nonlinear nature of the total PV curve makes these partial ones very difficult to interpret. In the present study, we describe a standardized way to measure the total PV curve. We have then tested the ability of these curves to detect changes in mouse lung structure in two common lung pathologies, emphysema and fibrosis. Results showed significant changes in several variables consistent with expected structural changes with these pathologies. This measurement of the lung PV curve in mice thus provides a straightforward means to monitor the progression of the pathophysiologic changes over time and the potential effect of therapeutic procedures.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/52376
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-27
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Lung Diseases Physiopathology Lung Volume Measurements Lung Physiology Animals Disease Models, Animal Emphysema Diagnosis Mice Pulmonary Fibrosis Swine Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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