NDLI: Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.

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Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.

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Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Afzal, Muhammad Z. Strande, Jennifer L.
Description	Author Affiliation: Afzal MZ ( Department of Medicine, Medical College of Wisconsin.); Strande JL ( Department of Medicine, Medical College of Wisconsin)
Abstract	Dystrophic cardiomyopathy is a poorly understood consequence of muscular dystrophy. Generating induced Pluripotent Stem Cells (iPSCs) from patients with muscular dystrophy is an invaluable cellular source for in vitro disease model systems and can be used for drug screening studies. Patient-derived urine cells have been used in successful reprogramming into induced pluripotent stem cells in order to model dystrophic cardiomyopathy(1). Addressing the safety concerns of integrating vector systems, we present a protocol using a non-integrating Sendai virus vector for transduction of Yamanaka factors into urine cells collected from patients with muscular dystrophy. This protocol generates fully reprogrammed clones within 2-3 weeks. The pluripotent cells are vector-free by passage-13. These dystrophic iPSCs can be differentiated into cardiomyocytes and used either to study disease mechanisms or for drug screening.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/52032
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-28
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Cellular Reprogramming Techniques Induced Pluripotent Stem Cells Cytology Muscular Dystrophies Pathology Urine Cell Differentiation Physiology Cellular Reprogramming Genetic Vectors Genetics Myocytes, Cardiac Sendai Virus Transduction, Genetic Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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