NDLI: An innovative method for exosome quantification and size measurement.

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Generation of genomic deletions in mammalian cell lines via CRISPR/Cas9.

Subcloning plus insertion (SPI)--a novel recombineering method for the rapid construction of gene targeting vectors.

Polyacrylamide gels for invadopodia and traction force assays on cancer cells.

A novel vertebral stabilization method for producing contusive spinal cord injury.

Green fluorescent protein-based expression screening of membrane proteins in Escherichia coli.

An ex vivo model to study hormone action in the human breast.

Phenotyping mouse pulmonary function in vivo with the lung diffusing capacity.

In vitro aggregation assays using hyperphosphorylated tau protein.

Analyzing the effects of stromal cells on the recruitment of leukocytes from flow.

Assessment of viability of human fat injection into nude mice with micro-computed tomography.

Chemotherapy-induced vascular toxicity--real-time in vivo imaging of vessel impairment.

Application of fluorescent nanoparticles to study remodeling of the endo-lysosomal system by intracellular bacteria.

Use of MALDI-TOF mass spectrometry and a custom database to characterize bacteria indigenous to a unique cave environment (Kartchner Caverns, AZ, USA).

High throughput characterization of adult stem cells engineered for delivery of therapeutic factors for neuroprotective strategies.

Surgical fixation of sternal fractures: preoperative planning and a safe surgical technique using locked titanium plates and depth limited drilling.

Evaluation of stem cell properties in human ovarian carcinoma cells using multi and single cell-based spheres assays.

Fluorescence-quenching of a liposomal-encapsulated near-infrared fluorophore as a tool for in vivo optical imaging.

Simple method for fluorescence DNA in situ hybridization to squashed chromosomes.

Investigating the spreading and toxicity of prion-like proteins using the metazoan model organism C. elegans.

Measuring protein stability in living zebrafish embryos using fluorescence decay after photoconversion (FDAP).

Optimized ex-ovo culturing of chick embryos to advanced stages of development.

Direct visualization of the murine dorsal cochlear nucleus for optogenetic stimulation of the auditory pathway.

Induction of protein deletion through in utero electroporation to define deficits in neuronal migration in transgenic models.

Preparation and characterization of SDF-1 -chitosan-dextran sulfate nanoparticles.

Measurement of smooth muscle function in the isolated tissue bath-applications to pharmacology research.

A fish-feeding laboratory bioassay to assess the antipredatory activity of secondary metabolites from the tissues of marine organisms.

Detection of in situ protein-protein complexes at the Drosophila larval neuromuscular junction using proximity ligation assay.

Generation of induced pluripotent stem cells from muscular dystrophy patients: efficient integration-free reprogramming of urine derived cells.

Reconstitution of a transmembrane protein, the voltage-gated ion channel, KvAP, into giant unilamellar vesicles for microscopy and patch clamp studies.

Environmental modulations of the number of midbrain dopamine neurons in adult mice.

Signal attenuation as a rat model of obsessive compulsive disorder.

Toxicological assays for testing effects of an epigenetic drug on development, fecundity and survivorship of malaria mosquitoes.

Production and use of lentivirus to selectively transduce primary oligodendrocyte precursor cells for in vitro myelination assays.

Detection of exosomal biomarker by electric field-induced release and measurement (EFIRM).

High-definition Fourier Transform Infrared (FT-IR) spectroscopic imaging of human tissue sections towards improving pathology.

Expression of fluorescent proteins in Branchiostoma lanceolatum by mRNA injection into unfertilized oocytes.

Electroporation of functional bacterial effectors into mammalian cells.

Isolation and enrichment of human adipose-derived stromal cells for enhanced osteogenesis.

Nucleocapsid Annealing-Mediated Electrophoresis (NAME) assay allows the rapid identification of HIV-1 nucleocapsid inhibitors.

Adult mouse venous hypertension model: common carotid artery to external jugular vein anastomosis.

Methods to evaluate cytotoxicity and immunosuppression of combustible tobacco product preparations.

Derivation of cardiac progenitor cells from embryonic stem cells.

A mouse fetal skin model of scarless wound repair.

Isolation of myofibroblasts from mouse and human esophagus.

Isolated hepatic perfusion as a treatment for liver metastases of uveal melanoma.

An innovative method for exosome quantification and size measurement.

Electrophysiological and morphological characterization of neuronal microcircuits in acute brain slices using paired patch-clamp recordings.

Direct induction of human neural stem cells from peripheral blood hematopoietic progenitor cells.

Isolation and quantitative immunocytochemical characterization of primary myogenic cells and fibroblasts from human skeletal muscle.

Automated measurement of pulmonary emphysema and small airway remodeling in cigarette smoke-exposed mice.

Investigating mast cell secretory granules; from biosynthesis to exocytosis.

A modified precipitation method to isolate urinary exosomes.

A surgical procedure for resecting the mouse rib: a model for large-scale long bone repair.

Isolation and immortalization of patient-derived cell lines from muscle biopsy for disease modeling.

Porous silicon microparticles for delivery of siRNA therapeutics.

Candida albicans biofilm development on medically-relevant foreign bodies in a mouse subcutaneous model followed by bioluminescence imaging.

In vivo optogenetic stimulation of the rodent central nervous system.

Measurement of the pressure-volume curve in mouse lungs.

Large-scale zebrafish embryonic heart dissection for transcriptional analysis.

In vitro functional characterization of mouse colorectal afferent endings.

Performing subretinal injections in rodents to deliver retinal pigment epithelium cells in suspension.

Scalable high throughput selection from phage-displayed synthetic antibody libraries.

Electrochemically and bioelectrochemically induced ammonium recovery.

Do's and don'ts of cryo-electron microscopy: a primer on sample preparation and high quality data collection for macromolecular 3D reconstruction.

Live-imaging of the Drosophila pupal eye.

Culture of embryonic mouse cochlear explants and gene transfer by electroporation.

Understanding early organogenesis using a simplified in situ hybridization protocol in Xenopus.

An innovative method for exosome quantification and size measurement.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Mehdiani, Arash Maier, Anatol Pinto, Antonio Barth, Mareike Akhyari, Payam Lichtenberg, Artur
Description	Author Affiliation: Mehdiani A ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf.); Maier A ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf.); Pinto A ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf.); Barth M ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf.); Akhyari P ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf); Lichtenberg A ( Research Group Experimental Surgery, Department of Cardiovascular Surgery, Medical Faculty, Heinrich-Heine-University Dusseldorf.)
Abstract	Although the biological importance of exosomes has recently gained an increasing amount of scientific and clinical attention, much is still unknown about their complex pathways, their bioavailability and their diverse functions in health and disease. Current work focuses on the presence and the behavior of exosomes (in vitro as well as in vivo) in the context of different human disorders, especially in the fields of oncology, gynecology and cardiology. Unfortunately, neither a consensus regarding a gold standard for exosome isolation exists, nor is there an agreement on such a method for their quantitative analysis. As there are many methods for the purification of exosomes and also many possibilities for their quantitative and qualitative analysis, it is difficult to determine a combination of methods for the ideal approach. Here, we demonstrate nanoparticle tracking analysis (NTA), a semi-automated method for the characterization of exosomes after isolation from human plasma by ultracentrifugation. The presented results show that this approach for isolation, as well as the determination of the average number and size of exosomes, delivers reproducible and valid data, as confirmed by other methods, such as scanning electron microscopy (SEM).
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50974
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-17
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Exosomes Chemistry Nanoparticles Ultracentrifugation Blood Chemical Analysis Microscopy, Electron, Scanning Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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