NDLI: A modified precipitation method to isolate urinary exosomes.

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A modified precipitation method to isolate urinary exosomes.

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A modified precipitation method to isolate urinary exosomes.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kanchi Ravi, Rupesh Khosroheidari, Mahdieh DiStefano, Johanna K.
Description	Author Affiliation: Kanchi Ravi R ( Diabetes, Cardiovascular and Metabolic Diseases Division, Translational Genomics Research Institute (TGen).); Khosroheidari M ( Diabetes, Cardiovascular and Metabolic Diseases Division, Translational Genomics Research Institute (TGen).); DiStefano JK ( Diabetes, Cardiovascular and Metabolic Diseases Division, Translational Genomics Research Institute (TGen))
Abstract	Identification of biomarkers that allow early detection of kidney diseases in urine and plasma has been an area of active interest for several years. Urinary exosome vesicles, 40-100 nm in size, are released into the urine under normal conditions by cells from all nephron segments and may contain protein, mRNA and microRNA representative of their cell type of origin. Under conditions of renal dysfunction or injury, exosomes may contain altered proportions of these components, which may serve as biomarkers for disease. There are currently several methods available for isolation of urinary exosomes, and we have previously conducted an experimental comparison of each of these approaches, including three based on ultracentrifugation, one using a nanomembrane ultrafiltration concentrator, one using a commercial precipitation reagent and one using a modification of the precipitation technique using ExoQuick reagent that we developed in our laboratory. We found the modified precipitation method produced the highest yield of exosome particles, miRNA, and mRNA, making this approach suitable for the isolation of exosomes for subsequent RNA profiling. We conclude that the modified exosome precipitation method offers a quick, scalable, and effective alternative for the isolation of exosomes from urine. In this report, we describe our modified precipitation technique using ExoQuick reagent for isolating exosomes from human urine.
File Format	HTM / HTML
e-ISSN	1940087X
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-16
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Exosomes Chemistry Fractional Precipitation Micrornas Urine Rna, Messenger Biological Markers Isolation & Purification Ultracentrifugation Ultrafiltration Urinalysis Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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