NDLI: Direct visualization of the murine dorsal cochlear nucleus for optogenetic stimulation of the auditory pathway.

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Direct visualization of the murine dorsal cochlear nucleus for optogenetic stimulation of the auditory pathway.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kozin, Elliott D. Darrow, Keith N. Hight, Ariel E. Lehmann, Ashton E. Kaplan, Alyson B. Brown, M. Christian Lee, Daniel J.
Description	Author Affiliation: Kozin ED ( Department of Otology and Laryngology, Harvard Medical School); Darrow KN ( Department of Communication Sciences and Disorders, Worcester State University.); Hight AE ( Department of Otology and Laryngology, Harvard Medical School.); Lehmann AE ( Department of Otology and Laryngology, Harvard Medical School.); Kaplan AB ( Department of Otology and Laryngology, Harvard Medical School.); Brown MC ( Department of Otology and Laryngology, Harvard Medical School.); Lee DJ ( Department of Otology and Laryngology, Harvard Medical School.)
Abstract	Investigation into the use of virus-mediated gene transfer to arrest or reverse hearing loss has largely been relegated to the peripheral auditory system. Few studies have examined gene transfer to the central auditory system. The dorsal cochlear nucleus (DCN) of the brainstem, which contains second order neurons of the auditory pathway, is a potential site for gene transfer. In this protocol, a technique for direct and maximal exposure of the murine DCN via a posterior fossa approach is demonstrated. This approach allows for either acute or survival surgery. Following direct visualization of the DCN, a host of experiments are possible, including injection of opsins into the cochlear nucleus and subsequent stimulation by an optical fiber coupled to a blue light laser. Other neurophysiology experiments, such as electrical stimulation and neural injector tracings are also feasible. The level of visualization and the duration of stimulation achievable make this approach applicable to a wide range of experiments.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/52426
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-20
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Auditory Pathways Physiology Cochlear Nucleus Surgery Animals Brain Stem Electric Stimulation Gene Transfer Techniques Mice Models, Animal Neurons Optogenetics Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Video-audio Media
Content Type	Text
Resource Type	Article

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