NDLI: Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.

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Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.

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Year: 2013 Issue: 72

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Simultaneous pre- and post-synaptic electrophysiological recording from Xenopus nerve-muscle co-cultures.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Yazejian, Bruce Yazejian, Rita M. Einarsson, Rachel Grinnell, Alan D.
Description	Country affiliation: United States Author Affiliation: Yazejian B ( Department of Physiology, David Geffen School of Medicine at UCLA, USA. yaz@ucla.edu)
Abstract	Much information about the coupling of presynaptic ionic currents with the release of neurotransmitter has been obtained from invertebrate preparations, most notably the squid giant synapse. However, except for the preparation described here, few vertebrate preparations exist in which it is possible to make simultaneous measurements of neurotransmitter release and presynaptic ionic currents. Embryonic Xenopus motoneurons and muscle cells can be grown together in simple culture medium at room temperature; they will form functional synapses within twelve to twenty-four hours, and can be used to study nerve and muscle cell development and synaptic interactions for several days (until overgrowth occurs). Some advantages of these co-cultures over other vertebrate preparations include the simplicity of preparation, the ability to maintain the cultures and work at room temperature, and the ready accessibility of the synapses formed. The preparation has been used widely to study the biophysical properties of presynaptic ion channels and the regulation of transmitter release. In addition, the preparation has lent itself to other uses including the study of neurite outgrowth and synaptogenesis, molecular mechanisms of neurotransmitter release, the role of diffusible messengers in neuromodulation, and in vitro synaptic plasticity.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/50253
Journal	Journal of Visualized Experiments
Issue Number	73
Language	English
Publisher	MyJove Corp.
Publisher Date	2013-03-11
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Motor Neurons Physiology Muscles Neuromuscular Junction Presynaptic Terminals Synapses Synaptic Potentials Animals Coculture Techniques Electrophysiological Phenomena Embryo, Nonmammalian Cytology Patch-clamp Techniques Xenopus Research Support, U.s. Gov't, Non-p.h.s. Video-audio Media
Content Type	Text
Resource Type	Article

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