NDLI: Direct induction of human neural stem cells from peripheral blood hematopoietic progenitor cells.

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Direct induction of human neural stem cells from peripheral blood hematopoietic progenitor cells.

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Direct induction of human neural stem cells from peripheral blood hematopoietic progenitor cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Wang, Tongguang Choi, Elliot Monaco, Maria Chiara G. Major, Eugene O. Medynets, Marie Nath, Avindra
Description	Author Affiliation: Wang T ( Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health); Choi E ( Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health.); Monaco MC ( Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health.); Major EO ( Laboratory of Molecular Medicine and Neuroscience, National Institute of Neurological Disorders and Stroke, National Institutes of Health.); Medynets M ( Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health.); Nath A ( Translational Neuroscience Center, National Institute of Neurological Disorders and Stroke, National Institutes of Health.)
Abstract	Human disease specific neuronal cultures are essential for generating in vitro models for human neurological diseases. However, the lack of access to primary human adult neural cultures raises unique challenges. Recent developments in induced pluripotent stem cells (iPSC) provides an alternative approach to derive neural cultures from skin fibroblasts through patient specific iPSC, but this process is labor intensive, requires special expertise and large amounts of resources, and can take several months. This prevents the wide application of this technology to the study of neurological diseases. To overcome some of these issues, we have developed a method to derive neural stem cells directly from human adult peripheral blood, bypassing the iPSC derivation process. Hematopoietic progenitor cells enriched from human adult peripheral blood were cultured in vitro and transfected with Sendai virus vectors containing transcriptional factors Sox2, Oct3/4, Klf4, and c-Myc. The transfection results in morphological changes in the cells which are further selected by using human neural progenitor medium containing basic fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). The resulting cells are characterized by the expression for neural stem cell markers, such as nestin and SOX2. These neural stem cells could be further differentiated to neurons, astroglia and oligodendrocytes in specified differentiation media. Using easily accessible human peripheral blood samples, this method could be used to derive neural stem cells for further differentiation to neural cells for in vitro modeling of neurological disorders and may advance studies related to the pathogenesis and treatment of those diseases.
File Format	HTM / HTML
e-ISSN	1940087X
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-28
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Hematopoietic Stem Cells Cytology Induced Pluripotent Stem Cells Neural Stem Cells Antigens, Cd34 Blood Astrocytes Cell Differentiation Physiology Culture Media Fibroblast Growth Factor 2 Nestin Biosynthesis Neurons Oligodendroglia Transcription Factors Metabolism Vascular Endothelial Growth Factor A Research Support, N.i.h., Intramural Video-audio Media
Content Type	Text
Resource Type	Article

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