NDLI: Phenotyping mouse pulmonary function in vivo with the lung diffusing capacity.

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Phenotyping mouse pulmonary function in vivo with the lung diffusing capacity.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Limjunyawong, Nathachit Fallica, Jonathan Ramakrishnan, Amritha Datta, Kausik Gabrielson, Matthew Horton, Maureen Mitzner, Wayne
Description	Author Affiliation: Limjunyawong N ( Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health.); Fallica J ( Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health.); Ramakrishnan A ( Department of International Health, Johns Hopkins University Bloomberg School of Public Health.); Datta K ( Department of Medicine, Johns Hopkins University School of Medicine.); Gabrielson M ( Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health.); Horton M ( Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health.); Mitzner W ( Department of Environmental Health Sciences, Johns Hopkins University Bloomberg School of Public Health)
Abstract	The mouse is now the primary animal used to model a variety of lung diseases. To study the mechanisms that underlie such pathologies, phenotypic methods are needed that can quantify the pathologic changes. Furthermore, to provide translational relevance to the mouse models, such measurements should be tests that can easily be done in both humans and mice. Unfortunately, in the present literature few phenotypic measurements of lung function have direct application to humans. One exception is the diffusing capacity for carbon monoxide, which is a measurement that is routinely done in humans. In the present report, we describe a means to quickly and simply measure this diffusing capacity in mice. The procedure involves brief lung inflation with tracer gases in an anesthetized mouse, followed by a 1 min gas analysis time. We have tested the ability of this method to detect several lung pathologies, including emphysema, fibrosis, acute lung injury, and influenza and fungal lung infections, as well as monitoring lung maturation in young pups. Results show significant decreases in all the lung pathologies, as well as an increase in the diffusing capacity with lung maturation. This measurement of lung diffusing capacity thus provides a pulmonary function test that has broad application with its ability to detect phenotypic structural changes with most of the existing pathologic lung models.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/52216
Journal	Journal of Visualized Experiments
Issue Number	95
Language	English
Publisher	MyJove Corp.
Publisher Date	2015-01-06
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Lung Diseases Diagnosis Physiopathology Pulmonary Diffusing Capacity Respiratory Function Tests Animals Mice Mice, Inbred C57bl Phenotype Research Support, N.i.h., Extramural Video-audio Media
Content Type	Text
Resource Type	Article

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