NDLI: Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology

Content Provider	PubMed Central
Author	Ge, Jingping Zhao, Jingwen Zhang, Luyan Zhang, Mengyun Ping, Wenxiang
Editor	Driessen, Arnold
Copyright Year	2014
Abstract	Double labeling of resistance markers and report genes can be used to breed engineered Saccharomyces cerevisiae strains that can assimilate xylose and glucose as a mixed carbon source for ethanol fermentation and increased ethanol production. In this study Saccharomyces cerevisiae W5 and Candida shehatae 20335 were used as parent strains to conduct protoplast fusion and the resulting fusants were screened by double labeling. High performance liquid chromatography (HPLC) was used to assess the ethanol yield following the fermentation of xylose and glucose, as both single and mixed carbon sources, by the fusants. Interestingly, one fusant (ZLYRHZ7) was demonstrated to have an excellent fermentation performance, with an ethanol yield using the mixed carbon source of 0.424 g g−1, which compares with 0.240 g g−1 (W5) and 0.353 g g−1 (20335) for the parent strains. This indicates an improvement in the ethanol yield of 43.4% and 16.7%, respectively.
Related Links	http://dx.doi.org/10.1371/journal.pone.0108311
Starting Page	108311
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-09-30
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology (2014)

Construction and analysis of high-ethanol-producing fusants with co-fermentation ability through protoplast fusion and double labeling technology.

Ethanol production from cassava starch by protoplast fusants of Wickerhamomyces anomalus and Galactomyces candidum

Construction of Lactose-Assimilating and High-Ethanol-Producing Yeasts by Protoplast Fusion

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Formation of interspecies fusants of Agaricus bisporus and Agaricus bitorquis mushroom by protoplast fusion

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Construction and Analysis of High-Ethanol-Producing Fusants with Co-Fermentation Ability through Protoplast Fusion and Double Labeling Technology

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Ethanol production from cassava starch by protoplast fusants of Wickerhamomyces anomalus and Galactomyces candidum

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