NDLI: Genetic and Epigenetic Changes in Chromosomally Stable and Unstable Progeny of Irradiated Cells

Content Provider	PubMed Central
Author	Baulch, Janet E. Aypar, Umut Waters, Katrina M. Yang, Austin J. Morgan, William F.
Editor	Iwamoto, Kazuya
Copyright Year	2014
Abstract	Radiation induced genomic instability is a well-studied phenomenon, the underlying mechanisms of which are poorly understood. Persistent oxidative stress, mitochondrial dysfunction, elevated cytokine levels and epigenetic changes are among the mechanisms invoked in the perpetuation of the phenotype. To determine whether epigenetic aberrations affect genomic instability we measured DNA methylation, mRNA and microRNA (miR) levels in well characterized chromosomally stable and unstable clonally expanded single cell survivors of irradiation. While no changes in DNA methylation were observed for the gene promoters evaluated, increased LINE-1 methylation was observed for two unstable clones (LS12 and CS9) and decreased Alu element methylation was observed for the other two unstable clones (115 and Fe5.0–8). These relationships also manifested for mRNA and miR expression. mRNA identified for the LS12 and CS9 clones were most similar to each other (261 mRNA), while the 115 and Fe5.0–8 clones were more similar to each other, and surprisingly also similar to the two stable clones, 114 and 118 (286 mRNA among these four clones). Pathway analysis showed enrichment for pathways involved in mitochondrial function and cellular redox, themes routinely invoked in genomic instability. The commonalities between the two subgroups of clones were also observed for miR. The number of miR for which anti-correlated mRNA were identified suggests that these miR exert functional effects in each clone. The results demonstrate significant genetic and epigenetic changes in unstable cells, but similar changes are almost as equally common in chromosomally stable cells. Possible conclusions might be that the chromosomally stable clones have some other form of instability, or that some of the observed changes represent a sort of radiation signature and that other changes are related to genomic instability. Irrespective, these findings again suggest that a spectrum of changes both drive genomic instability and permit unstable cells to persist and proliferate.
Related Links	http://dx.doi.org/10.1371/journal.pone.0107722
Starting Page	107722
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-09-24
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Epigenetic phenotypes distinguish microsatellite-stable and -unstable colorectal cancers

Chromosomally unstable mouse tumours have genomic alterations similar to diverse human cancers

Assembly of Rickettsia tsutsugamushi progeny in irradiated L cells.

Cancer Stem Cells in Colorectal Cancer: Genetic and Epigenetic Changes

Cryopreservation Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges

Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells

DNA alterations detected in the progeny of paternally irradiated Japanese medaka fish (Oryzias latipes).

A transcriptional and metabolic signature of primary aneuploidy is present in chromosomally-unstable cancer cells and informs clinical prognosis

Unstable molecules form stable tissues

Genetic and Epigenetic Changes in Chromosomally Stable and Unstable Progeny of Irradiated Cells

Similar Documents

Epigenetic phenotypes distinguish microsatellite-stable and -unstable colorectal cancers

Chromosomally unstable mouse tumours have genomic alterations similar to diverse human cancers

Assembly of Rickettsia tsutsugamushi progeny in irradiated L cells.

Cancer Stem Cells in Colorectal Cancer: Genetic and Epigenetic Changes

Cryopreservation Causes Genetic and Epigenetic Changes in Zebrafish Genital Ridges

Cell cycle and radiosensitivity of progeny of irradiated primary cultured human hepatocarcinoma cells

DNA alterations detected in the progeny of paternally irradiated Japanese medaka fish (Oryzias latipes).

A transcriptional and metabolic signature of primary aneuploidy is present in chromosomally-unstable cancer cells and informs clinical prognosis

Unstable molecules form stable tissues

Genetic and Epigenetic Changes in Chromosomally Stable and Unstable Progeny of Irradiated Cells