NDLI: Comparative DNA Damage and Repair in Echinoderm Coelomocytes Exposed to Genotoxicants

Content Provider	PubMed Central
Author	El-bibany, Ameena H. Bodnar, Andrea G. Reinardy, Helena C.
Editor	Dupont, Sam
Copyright Year	2014
Abstract	The capacity to withstand and repair DNA damage differs among species and plays a role in determining an organism's resistance to genotoxicity, life history, and susceptibility to disease. Environmental stressors that affect organisms at the genetic level are of particular concern in ecotoxicology due to the potential for chronic effects and trans-generational impacts on populations. Echinoderms are valuable organisms to study the relationship between DNA repair and resistance to genotoxic stress due to their history and use as ecotoxicological models, little evidence of senescence, and few reported cases of neoplasia. Coelomocytes (immune cells) have been proposed to serve as sensitive bioindicators of environmental stress and are often used to assess genotoxicity; however, little is known about how coelomocytes from different echinoderm species respond to genotoxic stress. In this study, DNA damage was assessed (by Fast Micromethod) in coelomocytes of four echinoderm species (sea urchins Lytechinus variegatus, Echinometra lucunter lucunter, and Tripneustes ventricosus, and a sea cucumber Isostichopus badionotus) after acute exposure to H2O2 (0–100 mM) and UV-C (0–9999 J/m2), and DNA repair was analyzed over a 24-hour period of recovery. Results show that coelomocytes from all four echinoderm species have the capacity to repair both UV-C and H2O2-induced DNA damage; however, there were differences in repair capacity between species. At 24 hours following exposure to the highest concentration of H2O2 (100 mM) and highest dose of UV-C (9999 J/m2) cell viability remained high (>94.6±1.2%) but DNA repair ranged from 18.2±9.2% to 70.8±16.0% for H2O2 and 8.4±3.2% to 79.8±9.0% for UV-C exposure. Species-specific differences in genotoxic susceptibility and capacity for DNA repair are important to consider when evaluating ecogenotoxicological model organisms and assessing overall impacts of genotoxicants in the environment.
Related Links	http://dx.doi.org/10.1371/journal.pone.0107815
Starting Page	107815
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-09-17
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

DNA damage in different Eisenia andrei coelomocytes sub-populations after in vitro exposure to hydrogen peroxide

DNA damage and repair in telomeres: relation to aging.

Damage and Repair of DNA in 5-Bromodeoxyuridine-Labeled Chinese Hamster Cells Exposed to Fluorescent Light

Letter: Damage and repair of DNA in 5-bromodeoxyuridine-labeled Chinese hamster cells exposed to fluorescent light.

DNA damage and mutation in human cells exposed to nitric oxide in vitro.

Adaptive Response in Mice Exposed to 900 MHz Radiofrequency Fields: Primary DNA Damage

DNA damage and repair of human skin keratinocytes concurrently exposed to pyrene derivatives and UVA light

Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

Genetic polymorphisms of DNA repair genes and chromosomal damage in workers exposed to 1,3-butadiene

Comparative DNA Damage and Repair in Echinoderm Coelomocytes Exposed to Genotoxicants

Similar Documents

DNA damage in different Eisenia andrei coelomocytes sub-populations after in vitro exposure to hydrogen peroxide

DNA damage and repair in telomeres: relation to aging.

Damage and Repair of DNA in 5-Bromodeoxyuridine-Labeled Chinese Hamster Cells Exposed to Fluorescent Light

Letter: Damage and repair of DNA in 5-bromodeoxyuridine-labeled Chinese hamster cells exposed to fluorescent light.

DNA damage and mutation in human cells exposed to nitric oxide in vitro.

Adaptive Response in Mice Exposed to 900 MHz Radiofrequency Fields: Primary DNA Damage

DNA damage and repair of human skin keratinocytes concurrently exposed to pyrene derivatives and UVA light

Ancient Sturgeons Possess Effective DNA Repair Mechanisms: Influence of Model Genotoxicants on Embryo Development of Sterlet, Acipenser ruthenus

Genetic polymorphisms of DNA repair genes and chromosomal damage in workers exposed to 1,3-butadiene

Comparative DNA Damage and Repair in Echinoderm Coelomocytes Exposed to Genotoxicants