NDLI: Directed Differentiation of Embryonic Stem Cells Using a Bead-Based Combinatorial Screening Method

Content Provider	PubMed Central
Author	Marina, Tarunina Hernandez, Diana Johnson, Christopher J. Rybtsov, Stanislav Vidya, Ramathas Jeyakumar, Mylvaganam Watson, Thomas Hook, Lilian Alexander, Medvinsky Mason, Chris Choo, Yen
Editor	Cooney, Austin John
Copyright Year	2014
Abstract	We have developed a rapid, bead-based combinatorial screening method to determine optimal combinations of variables that direct stem cell differentiation to produce known or novel cell types having pre-determined characteristics. Here we describe three experiments comprising stepwise exposure of mouse or human embryonic cells to 10,000 combinations of serum-free differentiation media, through which we discovered multiple novel, efficient and robust protocols to generate a number of specific hematopoietic and neural lineages. We further demonstrate that the technology can be used to optimize existing protocols in order to substitute costly growth factors with bioactive small molecules and/or increase cell yield, and to identify in vitro conditions for the production of rare developmental intermediates such as an embryonic lymphoid progenitor cell that has not previously been reported.
Related Links	http://dx.doi.org/10.1371/journal.pone.0104301
Starting Page	104301
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-09-01
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Biochemistry, Genetics and Molecular Biology(all) Agricultural and Biological Sciences(all) Medicine(all) Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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