NDLI: Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions

Content Provider	PubMed Central
Author	Henkel, Sebastian G. Beek, Alexander Ter Steinsiek, Sonja Stagge, Stefan Bettenbrock, Katja Mattos, M. Joost Teixeira De Sauter, Thomas Oliver, Sawodny Ederer, Michael
Editor	Torres, Néstor V.
Copyright Year	2014
Abstract	For adaptation between anaerobic, micro-aerobic and aerobic conditions Escherichia coli's metabolism and in particular its electron transport chain (ETC) is highly regulated. Although it is known that the global transcriptional regulators FNR and ArcA are involved in oxygen response it is unclear how they interplay in the regulation of ETC enzymes under micro-aerobic chemostat conditions. Also, there are diverse results which and how quinones (oxidised/reduced, ubiquinone/other quinones) are controlling the ArcBA two-component system. In the following a mathematical model of the E. coli ETC linked to basic modules for substrate uptake, fermentation product excretion and biomass formation is introduced. The kinetic modelling focusses on regulatory principles of the ETC for varying oxygen conditions in glucose-limited continuous cultures. The model is based on the balance of electron donation (glucose) and acceptance (oxygen or other acceptors). Also, it is able to account for different chemostat conditions due to changed substrate concentrations and dilution rates. The parameter identification process is divided into an estimation and a validation step based on previously published and new experimental data. The model shows that experimentally observed, qualitatively different behaviour of the ubiquinone redox state and the ArcA activity profile in the micro-aerobic range for different experimental conditions can emerge from a single network structure. The network structure features a strong feed-forward effect from the FNR regulatory system to the ArcBA regulatory system via a common control of the dehydrogenases of the ETC. The model supports the hypothesis that ubiquinone but not ubiquinol plays a key role in determining the activity of ArcBA in a glucose-limited chemostat at micro-aerobic conditions.
Related Links	http://dx.doi.org/10.1371/journal.pone.0107640
Starting Page	107640
File Format	PDF
ISSN	19326203
e-ISSN	19326203
Journal	PLoS ONE
Issue Number	9
Volume Number	9
Language	English
Publisher	Public Library of Science
Publisher Date	2014-09-30
Access Restriction	Open
Rights Holder	Public Library of Science
Subject Keyword	Research in Higher Education
Content Type	Text
Resource Type	Article
Subject	Multidisciplinary

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Oxygen transport—1. Basic principles

Computational modeling analysis of mitochondrial superoxide production under varying substrate conditions and upon inhibition of different segments of the electron transport chain

Aerobic respiratory chain of Escherichia coli is not allowed to work in fully uncoupled mode.

EVIDENCE FOR THE LOCALIZATION OF PLASTOCYANIN IN THE ELECTRON-TRANSPORT CHAIN OF PHOTOSYNTHESIS

The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents.

A mutant of Escherichia coli fumarate reductase decoupled from electron transport.

Electron transport chain from glycerol 3-phosphate to nitrate in Escherichia coli.

Electron Transport Chain from Glycerol 3-Phosphate to Nitrate in Escherichia coli

Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.

Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions

Similar Documents

Oxygen transport—1. Basic principles

Computational modeling analysis of mitochondrial superoxide production under varying substrate conditions and upon inhibition of different segments of the electron transport chain

Aerobic respiratory chain of Escherichia coli is not allowed to work in fully uncoupled mode.

EVIDENCE FOR THE LOCALIZATION OF PLASTOCYANIN IN THE ELECTRON-TRANSPORT CHAIN OF PHOTOSYNTHESIS

The Ada protein acts as both a positive and a negative modulator of Escherichia coli's response to methylating agents.

A mutant of Escherichia coli fumarate reductase decoupled from electron transport.

Electron transport chain from glycerol 3-phosphate to nitrate in Escherichia coli.

Electron Transport Chain from Glycerol 3-Phosphate to Nitrate in Escherichia coli

Sequence of b cytochromes relative to ubiquinone in the electron transport chain of Escherichia coli.

Basic Regulatory Principles of Escherichia coli's Electron Transport Chain for Varying Oxygen Conditions