NDLI: An integrated microfluidic system for screening of peptides specific to colon cancer cells and colon cancer stem cells using the phage display technology

Content Provider	IEEE Xplore Digital Library
Author	Yu-Jui Che Huei-Wen Wu Lein-Yu Hung Hwan-You Chang Kuan Wang Gwo-Bin Lee
Copyright Year	2014
Description	Author affiliation: Inst. of Mol. Med., Nat. Tsing Hua Univ., Hsinchu, Taiwan (Hwan-You Chang) \|\| Dept. of Power Mech. Eng., Nat. Tsing Hua Univ., Hsinchu, Taiwan (Yu-Jui Che; Huei-Wen Wu; Lein-Yu Hung; Gwo-Bin Lee) \|\| Inst. of Biol. Chem., Acad. Sinica, Taipei, Taiwan (Kuan Wang)
Abstract	Reagents binding specifically to target molecules are essential tools for clinical diagnosis and targeted therapy. Screening of target cell-surface specific affinity reagents with bench-top methods has some drawbacks, including time-consuming, labor-intensive and requirement of large-scale instrument. Microfluidic platforms may overcome these drawbacks because they could automate and complete the screening process within a shorter period of time. Phage display is a promising technology in selection of cell-surface specific peptides. In recent years, a subpopulation of tumor cells named cancer stem cells is believed to be the tumorigenic cells and closely associated with metastasis. A specific peptide that can recognize and differentiate cancer stem cells from the rest of cancer cell population is therefore useful for early diagnosis and targeted therapy. In this study, selection of M13 phage displayed peptides that bind with colon cancer cells and colon cancer stem cells using an integrated microfluidic system was successfully demonstrated. Compared with the traditional methods, the total selection process was shortened to 36 hours while traditional method needs almost a month. More importantly, the screening process can be automated and performed on a single microfluidic chip. The developed technique may be promising for early diagnosis of cancer and target therapeutics.
Starting Page	440
Ending Page	443
File Size	857664
Page Count	4
File Format	PDF
ISBN	9781479947263
DOI	10.1109/NEMS.2014.6908845
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2014-04-13
Publisher Place	USA
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Peptides Microfluidics Cancer Colon Stem cells Force Libraries biomarker microfluidics colon cancer cancer stem cell phage display library screening
Content Type	Text
Resource Type	Article

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

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