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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Sathuluri, R.R. Kitamura, M. Yamamura, S. Tamiya, E. |
| Copyright Year | 2006 |
| Description | Author affiliation: Toyama New Ind. Organ., Toyama (Sathuluri, R.R.) |
| Abstract | In this study, we report development of novel compartmental microfluidic chip devices for multiplexed single-cell sorting and analysis of antigen-specific single B-cells before and after stimulation with an antigen from a bulk cell suspension in the aqueous compartments that are generated by the two-phase liquid systems in the Y-shaped microchannels made from poly (dimethylsiloxane) (PDMS) polymer. Microfabrication technology allowed us to build the microfluidic device, which established the stable and high-throughput picoliter compartments of two-phase liquids under pressure-driven force due to viscosity and interfacial tension of liquids. Optimization of compartmental microfluidics revealed they could generate over ~30 compartments per sec with a size of < 150 pl volume suggest they are best suitable for single molecule and single-cell assays. This chip system has the ability of sorting up to ~1250 cells <100 sec. These devices are best suitable for high-sensitive on-chip bioassays since rapid mixing is possible in the compartments, hence this system serve as a micro total analysis system in diagnostic applications etc. |
| Starting Page | 642 |
| Ending Page | 645 |
| File Size | 2617085 |
| Page Count | 4 |
| File Format | |
| ISBN | 1424403758 |
| ISSN | 19300395 |
| DOI | 10.1109/ICSENS.2007.355550 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2006-10-22 |
| Publisher Place | South Korea |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Microfluidics Sorting Diseases Petroleum Cities and towns Materials science and technology Microchannel Liquids Viscosity Clinical diagnosis |
| Content Type | Text |
| Resource Type | Article |
| Subject | Electrical and Electronic Engineering |
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