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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Yaw-Jen Chang Cheng-Hao Chang Chih-Yu Hu Chia-Chen Liao |
| Copyright Year | 2006 |
| Description | Author affiliation: Chung Yuan Christian Univ., Chung Li (Yaw-Jen Chang; Cheng-Hao Chang; Chih-Yu Hu; Chia-Chen Liao) |
| Abstract | In this study, a Ni-Co alloy coated protein chip was fabricated by means of electrodepositing nickel and cobalt on the substrate surface. The protein was immobilized on the coated surface by the affinity mechanism of nickel and cobalt which belong to the intermediate metal group. Then, the immunoreaction can be performed on the chip. The results show that the alloy coating belongs to abnormal codeposition of Ni-Co alloy. The proportion of Ni-Co composition will affect the affinity of electroplated layer with protein and, consequentially, influence the results of immunoassay. Thus, in this paper the Taguchi method was adopted to determine the optimal fabrication process of the Ni-Co alloy coated chip using L18 orthogonal array. The experimental factors that affect the performance of abnormal codeposition include the quantity of cobalt, current density of electroplating, operation temperature, and pH of electroplating buffer. The major purpose of this research is the planarization of IMAC technique so that biological molecular can be absorbed on protein chip for further analysis. The advantages of this novel protein chip include good performance, high repeatability, and less cost. |
| Starting Page | 117 |
| Ending Page | 120 |
| File Size | 4033785 |
| Page Count | 4 |
| File Format | |
| ISBN | 1424403758 |
| ISSN | 19300395 |
| DOI | 10.1109/ICSENS.2007.355732 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2006-10-22 |
| Publisher Place | South Korea |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Fabrication Proteins Immune system Cobalt alloys Nickel alloys Coatings Current density Temperature Planarization Biological information theory |
| Content Type | Text |
| Resource Type | Article |
| Subject | Electrical and Electronic Engineering |
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