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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Ki Yong Choi Duck Kyun Choi Ga Young Han Dae Sung Yoon Tae Song Kim Sang Myung Lee Yoon-Sik Lee |
| Copyright Year | 2006 |
| Description | Author affiliation: Hanyang Univ., Seoul (Ki Yong Choi; Duck Kyun Choi) |
| Abstract | The sensitivity of immunoassays strongly depends on the binding affinity of the antibodies, which is also limited by a variety of other factors that are specific to the assay configuration, the type of label and instrument system used for detection. Magnetic beads are used in order to increase sensitivity in diagnosis system using the piezoelectric microcantilever. Use of magnetic beads is seemingly viable to insure the high sensitivity as well as the stable immunoassay. The ultra-sensitive magneto-mechanic detection of protein is accomplished by monitoring resonant frequency change of a cantilever functionalized with magnetic beads. The resonant frequency is surely shifted by mass and magnetic field effect. As a result, we practiced sandwich assay using CRP secondary antibody-coated magnetic bead on cantilever. Resonant frequency of the cantilever is changed by mass effect. When CRP secondary antibody-coated magnetic beads are attached to a CRP antigen, the changes in normalized resonant frequency shift $deltaf/f_{1}$ were found to definitely increase about 30 times. |
| Starting Page | 148 |
| Ending Page | 151 |
| File Size | 4032144 |
| Page Count | 4 |
| File Format | |
| ISBN | 1424403758 |
| ISSN | 19300395 |
| DOI | 10.1109/ICSENS.2007.355740 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2006-10-22 |
| Publisher Place | South Korea |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Magnetosphere Magnetic resonance Biosensors Immune system Resonant frequency Chemical and biological sensors Instruments Magnetic sensors Sensor systems Frequency conversion |
| Content Type | Text |
| Resource Type | Article |
| Subject | Electrical and Electronic Engineering |
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