NDLI: Effects of E. coil resistant mutation on phage lysis

Content Provider	IEEE Xplore Digital Library
Author	Hong-ming Li Zhi-xin Cui Xue-qin Wan Hong Jiang Shu-yi Tan Xia Li Wei-xia Diao Wan-kelan Li Su-yang Wang
Copyright Year	2011
Description	Author affiliation: Medical College & Foshan Stomatology Hospital, Foshan University, HEBIN road, Foshan, 5th, P.R. China (Hong-ming Li; Zhi-xin Cui; Xue-qin Wan; Hong Jiang; Shu-yi Tan; Xia Li; Wei-xia Diao) \|\| Northeastern University, Shenyang, P.R. China (Wan-kelan Li; Su-yang Wang)
Abstract	To investigate the effects on its virulent phage lysis of Escherichia coli resistant mutation. Methods First, isolate the chloramphenicol-sensitive strains and resistant strains from the excrement samples. Then, isolate the virulent phage lysis using the sensitive strains as the host bacteria and extracted the resistant plasmids of the resistant strains to the chloramphenicol resistant transformation experiment to obtain mutant strains. Finally, examine the sensitivity of the sensitive strains, resistant strains and mutant strains. Result The sensitive strains and the mutant strains are sensitive to the phage and has lysis effects while the resistant strains are insensitive and have no lysis. Conclusion The lysis of E. coli phage has no direct relationships with the resistant character and has strain specificity. The mutant strains(sensitive strains with chloramphenicol resistance) still have sensitivity.
Starting Page	1296
Ending Page	1299
File Size	1066441
Page Count	4
File Format	PDF
ISBN	9781612847238
e-ISBN	9781612847269
DOI	10.1109/HHBE.2011.6029069
Language	English
Publisher	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Publisher Date	2011-08-19
Publisher Place	China
Access Restriction	Subscribed
Rights Holder	Institute of Electrical and Electronics Engineers, Inc. (IEEE)
Subject Keyword	Drugs Escherichia coli Transforms Drug resistance Strain Sensitive strains Resistant strains Microorganisms Sensitivity Virulent phage Suspensions Mutant strains Immune system
Content Type	Text
Resource Type	Article

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