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| Content Provider | IEEE Xplore Digital Library |
|---|---|
| Author | Wang Cai Zhao Zhigang Zhao Kangjun Wang Chang Yu Ting Bao Hong Teng Chun Yan |
| Copyright Year | 2011 |
| Description | Author affiliation: Affiliated Hospital, Beihu University Jilin, China (Zhao Zhigang; Zhao Kangjun) || The Second Hospital of JiLin University, Chuangchun, China (Yu Ting; Bao Hong; Teng Chun Yan) || Medical Laboratory College, Beihua University, Jilin, China (Wang Cai) || Jilin City Central Hospital (Wang Chang) |
| Abstract | In this study, HIV was taken as the study object to establish a method for the detection of RNA viral pathogen causing unknown infectious disease. The serum from patients with acquired immune deficiency syndrome (AIDS) were filtered and treated with DNase to extract endogenous DNA using DNase-sequence-independent single primer amplification (SISP). Viral RNA from the serum was extracted, reverse transcripted, and the double-stranded cDNA was synthesized. The double-stranded cDNA was digested with Taq I and added to connect the adapter molecule, the pathogenic gene were amplified non-specifically with the adapter molecule as primers, then all the PCR products were sequenced and analyzed. The results showed that a number of exogenous DNA fragments were obtained using DNase-sequence-independent single primer amplification. The result that All PCR products were sequenced and analyzed verified that all sequences were consistent with those of the known pathogenic genes (HIV virus). In conclusion, a viral RNA pathogen can be determined using DNase-sequence-independent single primer amplification. |
| Starting Page | 1148 |
| Ending Page | 1151 |
| File Size | 397421 |
| Page Count | 4 |
| File Format | |
| ISBN | 9781612847238 |
| e-ISBN | 9781612847269 |
| DOI | 10.1109/HHBE.2011.6029029 |
| Language | English |
| Publisher | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Publisher Date | 2011-08-19 |
| Publisher Place | China |
| Access Restriction | Subscribed |
| Rights Holder | Institute of Electrical and Electronics Engineers, Inc. (IEEE) |
| Subject Keyword | Pathogens DNase-sequence-independent single primer amplification RNA Acquired immune deficiency syndrome DNA Human immunodeficiency virus Viruses (medical) AIDS virus Introduction Nfectious diseases |
| Content Type | Text |
| Resource Type | Article |
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