NDLI: Opto-Electric Biosensor to Examine In Vitro Toxicity Stimuli to Endothelial Cell Motility and Morphology

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Chang, K. Choi Margraves, Chuck Anthony, E. English Kenneth, D. Kihm
Copyright Year	2007
Abstract	An integrated opto-electric biosensor is developed that uses an optically transparent and electrically conductive indium tin oxide (ITO) thin film coated on a slide glass substrate. This biosensor can simultaneously acquire the micro-impedance response and microscopic images of live cells in vitro under various toxic agent stimuli. The dynamic response of live porcine pulmonary artery endothelial cells (PPAECs) exposed to various doses of cytochalasin D are comprehensively examined by monitoring the micro-impedance characteristics at a specified frequency and DICM images using the opto-electric biosensor. The change in PPAEC morphology and motility caused by cytochalasin D clearly illustrates the dose-dependent actin filament disruption where optical images are correlated with the changes in the micro-electric impedance.
Sponsorship	Bioengineering Division
Starting Page	61
Ending Page	62
Page Count	2
File Format	PDF
ISBN	0791847985
DOI	10.1115/SBC2007-176715
Conference Proceedings	ASME 2007 Summer Bioengineering Conference
Language	English
Publisher Date	2007-06-20
Publisher Place	Keystone, Colorado, USA
Access Restriction	Subscribed
Subject Keyword	Dynamic response Thin films Transparency Optical images Glass Biosensors Endothelial cells Pulmonary artery
Content Type	Text
Resource Type	Article

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Opto-Electric Biosensor to Examine In Vitro Toxicity Stimuli to Endothelial Cell Motility and Morphology