NDLI: A Sensitive Multiparametric Biosensor With Capabilities of Rapid Toxicity Detection of Drinking Water

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Liu, Fei Anis, N. Nordin Li, Fang Voiculescu, Ioana
Copyright Year	2013
Abstract	Recently, there has been interest to develop biosensors based on live mammalian cells to monitor the toxicity of water. The cell viability after exposure to toxic water can be monitored by electric cell-substrate impedance sensing (ECIS) of the cell membrane. However, these impedance based toxicity sensors can only provide one single sensing endpoint (impedance measurement), and many toxicants cannot be detected at the concentration between Military Exposure Guideline levels and estimated Human Lethal Concentrations. The goal of this paper is to provide a rapid and sensitive sensing platform for long-term water toxicity detection. In this paper a novel multiparametric biosensor with integrated microfluidic channels for water toxicity detection is presented. Toxicity tests to study bovine aortic endothelial cells (BAECs) responsiveness to health-threatening concentrations of ammonia in de-ionized (DI) water will be presented. We demonstrated the BAECs can rapidly respond to ammonia concentrations between the military exposure guideline of 2mM and human lethal concentration of 55mM. The successful testing of water toxicity by simultaneous gravimetric and impedimetric measurements indicates that the multiparametric biosensor platform is able to perform rapid and sensitive detection of water toxicity and minimize the false-positive rate.
File Format	PDF
ISBN	9780791856390
DOI	10.1115/IMECE2013-62281
Volume Number	Volume 10: Micro- and Nano-Systems Engineering and Packaging
Conference Proceedings	ASME 2013 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2013-11-15
Publisher Place	San Diego, California, USA
Access Restriction	Subscribed
Subject Keyword	Water Military systems Cell membranes Sensors Biosensors Endothelial cells Microfluidics Testing
Content Type	Text
Resource Type	Article

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in