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Content Provider | The American Society of Mechanical Engineers (ASME) Digital Collection |
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Author | Cho, Myoung-Ock Chang, Hyo Mi Yu, Yeon Gyu Han, Hwataik Kim, Jung Kyung |
Copyright Year | 2011 |
Abstract | There are several methods to detect asbestos including phase contrast microscopy (PCM), polarized light microscopy, X-ray diffraction, and electron microscopy. Although the PCM method is widely used due to its simple process and relatively low cost, it is a time-consuming and laborious process that is manually performed by a human counter. We developed a high-throughput microscopy (HTM) system for automated counting of airborne asbestos fibers to automate the conventional PCM method. Our results show that automatic image acquisition by synchronization of charge-coupled device (CCD) camera with movement of stages, and image analysis using image processing software, significantly reduced time consumption and labor. In this study, we used DksA chrysotile-adhesive protein for the selective detection of asbestos. DksA, known as the protein that specifically attaches to chrysotile, was extracted from Escherichia coli through a recombinant protein technique. We tried to detect chrysotile selectively from other fibers or particles, and we developed a highly selective and automated low-cost device for automated identification and enumeration of airborne asbestos fibers based on the HTM method. |
Starting Page | 407 |
Ending Page | 411 |
Page Count | 5 |
File Format | |
ISBN | 9780791854884 |
DOI | 10.1115/IMECE2011-63721 |
Volume Number | Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology |
Conference Proceedings | ASME 2011 International Mechanical Engineering Congress and Exposition |
Language | English |
Publisher Date | 2011-11-11 |
Publisher Place | Denver, Colorado, USA |
Access Restriction | Subscribed |
Subject Keyword | Phase contrast microscopes Image processing Computer software X-ray diffraction Electron microscopy Synchronization Fibers Proteins Adhesives Microscopy Polarization (light) Asbestos fibers Particulate matter |
Content Type | Text |
Resource Type | Article |
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