NDLI: High Flow Rate Device for Circulating Tumor Cell Capture

Content Provider	The American Society of Mechanical Engineers (ASME) Digital Collection
Author	Park, Taehyun Park, Daniel Sangwon Michael, C. Murphy
Copyright Year	2011
Abstract	Circulating tumor cells (CTCs) were captured at high flow rates with a high recovery rate using a small footprint disposable polymer micro device. A new concept of target cell capture was introduced to break through the barriers limiting current approaches. Several potential designs were parametrically simulated using computational fluid dynamics (CFD) to achieve the best performance. The high flow rate device (HFRD) was fabricated in polymethyl methacrylate (PMMA) based on simulation results. Antibodies (anti-EpCAM) were immobilized on the PMMA device with surface treatments including UV modification and amine functionalization. A novel rare cell sample preparation method was established to provide an exact number of initial target cells to accurately test the rare cell performance. The precisely prepared samples of rare target tumor cells were spiked in a solution containing human erythrocytes, with a 40% hematocrit. The mean recovery rate with the HFRD was 85% at a 750 μL/min flow rate.
Starting Page	413
Ending Page	416
Page Count	4
File Format	PDF
ISBN	9780791854884
DOI	10.1115/IMECE2011-63750
Volume Number	Volume 2: Biomedical and Biotechnology Engineering; Nanoengineering for Medicine and Biology
Conference Proceedings	ASME 2011 International Mechanical Engineering Congress and Exposition
Language	English
Publisher Date	2011-11-11
Publisher Place	Denver, Colorado, USA
Access Restriction	Subscribed
Subject Keyword	Ultraviolet radiation Simulation results Computational fluid dynamics Erythrocytes Polymers Flow (dynamics) Surface finishing Tumors
Content Type	Text
Resource Type	Article

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